〔Abstract〕 Objective To investigate the effect of acid-sensing ion channel 1(ASIC1) gene knockout on articular cartilage injury in adjuvant arthritis(AA) of mice. Methods Wild-type mice and ASIC1 knockout mice were divided into normal group and model group. Arthritis score was performed by observing the inflammation of paws, and the right paw swelling was measured. HE staining, immunohistochemistry, TUNEL and ELISA were used to detect the injury of articular cartilage and arthritic inflammation. Results The arthritis score and paw swelling of AA mice with ASIC1 knockout was lower than that of the AA wild-type mice. AA mice with ASIC1 knockout showed less destruction and increased expression of collagen-Ⅱin articular cartilage. Moreover, the lower apoptosis rate was observed by TUNEL assay in AA mice with ASIC1 knockout by comparing with AA wild-type mice. Furthermore, ELISA assay showed that the levels of IL-1β、TNF-α in the serum decreased in AA mice with ASIC1 knockout. Conclusion Knockout of ASIC1 may have protective effect on articular cartilage injury.