〔Abstract〕 Objective To analyze the expression and clinical prognostic significance of nuclear Dbf2-related kinase 1(NDR1) in hepatocellular carcinoma, and to investigate the biological function and regulatory mechanism ofNDR1in hepatocellular carcinoma cells. Methods ENCORI database was used to analyze the correlation ofNDR1and c-Myc. Cycloheximide(CHX) experiment analyzed the relationship betweenNDR1and c-Myc protein stability. The expression levels ofNDR1in liver cancer tissues and normal tissues and its relationship with the survival rate of liver cancer patients were analyzed using the ENCORI database. MYC-NDR1eukaryotic expression vector was constructed, transfected with hepatocellular carcinoma HepG2 cells, and its expression was verified by protein immuno blotting(Western blot); cell proliferation and migration ability were detected by CCK-8 assay, cell clone formation assay and scratch assay, respectively. The correlation betweenNDR1and c-Myc expression was analyzed using the ENCORI database, and the relationship betweenNDR1and c-Myc protein was investigated using a protein synthesis inhibitor CHX dosing assay. Results The results of the ENCORI database showed that the expression ofNDR1in liver cancer tissues was higher than that in normal tissues and the overall survival rate of patients with highNDR1expression was lower than that of patients with lowNDR1expression, and the difference was statistically significant(P<0.001). The results of the CCK-8 assay showed that the MYC-NDR1group grew faster than the empty vector group(P<0.001). The clone formation assay showed that the number of clones in the MYC-NDR1group was higher than that in the empty vector group(P<0.001). The cell scratch assay showed that the mean migration distance in the MYC-NDR1group was greater than that in the empty vector group(P<0.001). ENCORI database analysis showed thatNDR1correlated with c-Myc expression(R=0.184,P<0.001); CHX dosing assay showed that the reduction of c-Myc protein in the MYC-NDR1group was lower than that in the empty vector group during the same time. Conclusion NDR1is highly expressed in hepatocellular carcinoma tissues, closely correlated with poor prognosis of hepatocellular carcinoma patients, and positively correlated with the expression ofc-Mycgene. The study successfully constructes MYC-NDR1eukaryotic expression vector, and the expression product MYC-NDR1can increase the stability of c-Myc protein and promote the proliferation and migration of human hepatocellular carcinoma cells.