〔Abstract〕 Objective To analyze the pregnancy outcome of preimplantation genetic testing for aneuploidy(PGT-A) in cycles with different indications.Methods The clinical information of 549 couples who underwent PGT-A were retrospectively analyzed.The cycles were divided into 6 groups according to the indication for PGT-A,namely: recurrent pregnancy loss group(n=304),repeated implantation failure group(n=57),advanced age group(≥38years old,n=80),history of adverse pregnancy group(chorionic trisomy or adverse pregnancy,n=24),male factor infertility group(n=67),and abnormal sex chromosome number group(n=17).The basic information,the number of retrieved oocytes,embryo biopsy result and pregnancy outcome were compared among different indication groups.Results The average age and days of gonadotropin(Gn) used among the six groups were statistically different(P<0.001).The average number of retrieved oocytes,the rate of good-quality embryos,mosaic embryos,abnormal embryos and normal embryos,the average ovarian sensitivity index(OSI) among the six groups were statistically different(P=0.03,P<0.001,P=0.03,P<0.001,P<0.001,P<0.001).Advanced age group had the highest rate of abnormal embryos,the least average number of retrieved oocytes,the lowest OSI and the lowest rate of normal embryos.There were statistical differences in clinical pregnancy rate,ongoing pregnancy rate and cumulative pregnancy rate per oocyte retrieved cycle(P<0.001) among the six groups,but there were no statistical differences in clinical pregnancy rate,ongoing pregnancy rate per transfer cycle and cumulative pregnancy rate among the five groups except for the male factor infertility group.Conclusion PGT-A can detect euploid embryo to transfer thereby improving pregnancy efficiency.The advanced age women have normal embryo to transfer and can obtain a better pregnancy rate,which may shorten their time of “take-baby-home”.At the same time,PGT-A can significantly improve the pregnancy outcome of those with male factor infertility.