〔Abstract〕 Objective To investigate whether the cyclic adenosine monophosphate(cAMP)/exchange proteins directly activated by cAMP(Epac)/ras-related protein 1(Rap1) signalling pathway is involved in the intervening mechanisms of interleukin-1β(IL-1β), tumor necrosis factor-α(TNF-α), brain-derived neurotrophic factor(BDNF) and Jujuboside A(JuA) secretion by NG2 cells. Methods NG2 cells were cultured in vitro and the experiment was divided into control group,pertussis toxin( PTX) group,ESI-09 group,JuA group and positive drug group. The effect of different concentrations of JuA on the survival rate of NG2 cells was detected by CCK-8 method,and the expression of IL-1β,TNF-α,BDNF,cAMP,Epac,Rap1 mRNA and protein in each group was detected by RT-PCR and Western blot. Results Compared with the control group,the PTX group decreased the expression of IL-1β and TNF-α mRNA and protein( P<0. 01) and increased the expression of cAMP and BDNF mRNA and protein( P<0. 01); the ESI-09 group increased the expression of IL-1β and TNF-α mRNA and protein( P<0. 05) and decreased the expression of BDNF,Epac and Rap1 mRNA and protein expression( P<0. 01); the JuA group and positive drug group increased IL-1β,TNF-α,BDNF,cAMP,Epac,Rap1 mRNA and protein expression( P<0. 01). Conclusion The cAMP/Epac/Rap1 signaling pathway is involved in the secretion of IL-1β,TNF-α,and BDNF by NG2 cells. JuA may act on cAMP/Epac/Rap1 signaling pathway to affect the secretion of BDNF by NG2 cells.