〔Abstract〕 Objective To study the effect of Er, Cr: YSGG laser on the mineralization of dentin collagen fiber, accelerate the mineralization process and shorten the mineralization time. Methods Human health third molarswere selected to remove the teeth of the tooth enamel, degenerate the teeth, leave the dentin collagen fiber web, place the degenerative collagen fiber in the configured re-mineral fluid. Using Er, Cr: YSGG, the teeth before and after the test were used to analyze the surface morphology using SEM scanning electron microscopy, and the EDS X-ray energy spectrum was analyzed by the distribution of calcium phosphorus. Results Er, Cr: YSGG laser irradiation laser irradiated tooth in the teeth in the teeth, new minerals were generated, which covered the inner wall of the teeth, the calcium phosphorus element was enriched in the inner wall of the teeth. Conclusion YSGG laser can help dentin collagen fibers mineralized in mineralization fluid, form new minerals.This method can shorten the mineralization time of dentin collagen fibers in mineralization fluid, which is the re-ore.