〔Abstract〕 Objective To investigate the associations between the single nucleotide polymorphism(SNP) in vestigial like family member 4(VGLL4) gene and Helicobacter pylori(H. pylori) infection. Methods The blood samples of 450 normal physical examiners were collected, and the samples were divided into H. pylori negative group(n=220) and H. pylori positive group(n=230) using enzyme-linked immunosorbent assay(ELISA). SNP rs1803489, rs7617620, and rs13078528 in VGLL4 gene were genotyped using polymerase chain reaction(PCR)-restriction fragment length polymorphism(RFLP) technology. Results SNP rs1803489, rs7617620, and rs13078528 in VGLL4 gene were not associated with H. pylori infection in the Han population in Baotou, Inner Mongolia. Conclusion SNP rs1803489, rs7617620, and rs13078528 in VGLL4 gene may not play a major role in H. pylori infection in Baotou Han population.