〔Abstract〕 Objective To establish a three-dimensional cephalometric standardized value database for Anhui province, to study the craniofacial characteristics of normal adult population in Anhui, China and to provide effective information for making orthodontic and orthognathic surgery treatment plans of the craniofacial deformity. Methods 160 normal occlusal volunteers were selected according to the inclusive and exclusive criteria and were photographed by Cone Beam CT(CBCT). The coordinate system was established for the 3D model reconstructed with Dolphin based on CBCT data, and 38 hard tissue landmarks were located. 40 lines and 20 angles were defined and measured, and measurements were analyzed with SPSS 17.0, to evaluate craniofacial features of the overall, midface, maxilla, mandible, alveolar bone, and dentition of the standard population. Results The 3D cephalometric measurements of craniofacial hard tissues of normal adults in Anhui were obtained. The symmetry of craniofacial hard tissues was good both in males and females with the maximum asymmetry ratio of 2.2% and the minimum of 0.01%. The gender comparisons showed that most of lines measured of males were larger than those of females(P<0.01), while there was no significant difference in the measurements of the midline deviation of upper and lower mandible, the relative deviation of the maxillary and mandible and the protrusion of upper and lower incisor. Additionally, there was no statistical significance in angles measured between genders, except for the occlusal plane angle. The results also showed that Chinese had unique facial features based on the measured data, compared with other ethnic populations. Conclusion The normal occlusal and maxillofacial hard tissue of Anhui Han has good symmetry.The gender obviously influences line measurements, with generally the measurements of males larger than females, but it has no effect on most angle measurements.