〔Abstract〕 Objective By testing the tensile strength of the poly(vinyl alcohol)(PVA)/bacterial cellulose(BC) composite membrane and its effect on the proliferation of mouse embryonic fibroblasts, its potential as new bone tissue engineering membrane were studied. Methods PVA-BC films of different proportions and pure PVA films were prepared by self-evaporation method. The tensile strength of each group was tested. The group with the highest tensile strength was immersed in deionized water for 0.5 h to measure its wet tensile strength. The microstructure of pure PVA film and the film with the highest tensile strength was observed by scanning electron microscopy(SEM). X-ray diffraction and Fourier transform infrared(FTIR) spectroscopy were used to analyze pure PVA, pure BC, and the film with the highest tensile strength respectively. Cell counting kit-8(CCK-8) was applied to detect the survival rate in the blank control group, the pure PVA film group, and the composite film group with the highest tensile strength. Results PVA-BC composite films were successfully prepared, X-ray diffraction and FTIR analysis revealed the co-presence of PVA and bacterial cellulose in the composite film. The initial tensile strength of the composite membrane increased with the BC ratio. When the concentration ratio of PVA to BC was 10 ∶7, the tensile strength reached(155.5±14.7) MPa, and wet samples reached(13.8±1.2) MPa. The CCK-8 test of NIH/3 T3 showed that there was no significant difference among the PVA-BC composite film group, pure PVA group and blank control group after 1,4 and 7 days of cell culture(P>0.05). Conclusion PVA-BC film fabricated by blending method obtain certain mechanical properties and biocompatibility in both wet and dry states, which may be an appropriate candidate as a GBR membranes for clinical application.