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Objective By testing the tensile strength of the poly(vinyl alcohol)(PVA)/bacterial cellulose(BC) composite membrane and its effect on the proliferation of mouse embryonic fibroblasts, its potential as new bone tissue engineering membrane were studied. Methods PVA-BC films of different proportions and pure PVA films were prepared by self-evaporation method. The tensile strength of each group was tested. The group with the highest tensile strength was immersed in deionized water for 0.5 h to measure its wet tensile strength. The microstructure of pure PVA film and the film with the highest tensile strength was observed by scanning electron microscopy(SEM). X-ray diffraction and Fourier transform infrared(FTIR) spectroscopy were used to analyze pure PVA, pure BC, and the film with the highest tensile strength respectively. Cell counting kit-8(CCK-8) was applied to detect the survival rate in the blank control group, the pure PVA film group, and the composite film group with the highest tensile strength. Results PVA-BC composite films were successfully prepared, X-ray diffraction and FTIR analysis revealed the co-presence of PVA and bacterial cellulose in the composite film. The initial tensile strength of the composite membrane increased with the BC ratio. When the concentration ratio of PVA to BC was 10 ∶7, the tensile strength reached(155.5±14.7) MPa, and wet samples reached(13.8±1.2) MPa. The CCK-8 test of NIH/3 T3 showed that there was no significant difference among the PVA-BC composite film group, pure PVA group and blank control group after 1,4 and 7 days of cell culture(P>0.05). Conclusion PVA-BC film fabricated by blending method obtain certain mechanical properties and biocompatibility in both wet and dry states, which may be an appropriate candidate as a GBR membranes for clinical application.

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Objective To investigate the interaction between intracellular chloride ion protein 1(CLIC1) and activated protein kinase C receptor 1(RACK1). Methods Plasmids pcDNA3.1-RACK1-HA and/or pcDNA3.1-CLIC1-FLAG were transfected into HEK 293 T cells, and pcDNA3.1-RACK1-HA and/or pcDNA3.1-CLIC1-FLAG were transfected into COS7. GST-pulldown and immunoprecipitation assays were performed to determine the interaction between CLIC1 and RACK1in vivoandin vitro. The co-localization of CLIC1 and RACK1 was observed by indirect immunofluorescence assay. Results Western blot confirmed that CLIC1 and RACK1 could be highly expressed in HEK 293 T cells. GST-pulldown showed that RACK1 bound CLIC1in vitro, and immunoprecipitation showed that CLIC1 and RACK1 interactedin vivo. Furthermore, indirect immunofluorescence assay showed CLIC1 co-localized with RACK1. Conclusion Human CLIC1 protein interacts with RACK1in vitroandin vivo.

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Objective To explore the effect of wogonoside(WG)on theKrüppel-like factor4(KLF4)/nuclear factor κB(NF-κB) pathway of RAW264.7 macrophages subject to high glucose stimulation. Methods RAW264.7 macrophages were cultured and the concentration range of inactive effect of WG on cells was detected by CCK-8.Macrophages randomly grouped into LG group(medium for 5.5 mmol/L of glucose), D group(30 mmol/L mannitol medium), HG group(medium for 30 mmol/L glucose), LG+WG50 group(5.5 mmol/L glucose + 50 μmol/L WG), HG+WG12.5 group(30 mmol/L glucose+12.5 μmol/L WG), HG+WG25 group(30 mmol/L glucose+25 μmol/L WG), HG+WG50 group(30 mmol/L glucose+50 μmol/L WG). The expression of inducible nitric oxide synthase(iNOS) in cells of each group was determined by immunofluorescence. NF-κB p65, NF-κB pp65, KLF4, iNOS, interleukin-1β(IL-1β), tumor necrosis factor alpha(TNF-α) expression in each group was detected by Western blot. mRNA expression of KLF4, iNOS,IL-1β, TNF-α in each group was detected by qRT-PCR. IL-1β, TNF-α secretion of cells in each group was detected by ELISA. Results Compared with LG group, the expression of iNOS in HG group was higher(P<0.01), and the expression of iNOS in HG+W50 group was lower than that in HG group(P<0.01). Compared with the LG group, high glucose can inhibit KLF4 expression(P<0.01), stimulate NF-κB pp65, iNOS,IL-1β, TNF-α expression(P<0.01), and increase the IL-1β, TNF-α level in the supernatant(P<0.01); However, the inflammatory index in the WG groups significantly decreased(P<0.01), but expression of KLF4 dose-dependent increased(P<0.01). Conclusion WG inhibits the expression of inflammatory factors and associated proteins secreted by macrophages by high glucose, and has the therapeutic effect of improving the protective factor-KLF4.

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Objective To investigate the expression differences of helix-loop-helix hand domain family member D 1/2(EFhd1/2) in Alzheimer′s disease(AD) patients and model mice. Methods The expression changes of EFhd1/2 in AD patients were compared by using data from Alzheimer′s disease database(AlzData), and the changes in mRNA levels and protein levels of EFhd1/2 in brain tissues of AD patients and healthy control group were detected by qPCR and Western blot. The changes of EFhd1/2 mRNA and protein expression in brain tissues of AD models and wild-type mice of 3-month-old and 6-month-old were detected. Microglia were isolated from AD models and detected the changes of EFhd1/2 by RNA-sequencing. Results The Analysis of Alzheimer′s Disease Database data showed that the mRNA levels of EFhd1 in AD patients increased, while EFhd2 decreased. AD patients brain tissue samples showed an upward trend in the expression of EFhd1 in different brain regions of AD patients compared with healthy controls, while EFhd2 was not different. In the AD mice model, the mRNA levels of EFhd1 were similar in both 3-month-old and 6-month-old AD mice compared with wild-type mice, but the protein levels of EFhd1 increased; the mRNA levels of EFhd2 increased in 3-month-old AD mice, and protein levels remained similar in the brain tissue from AD mice aged 3 months and 6 months. However, EFhd2 increased in microglia from 6-month-old AD mice. Conclusion EFhd1 increased in both AD model mice and brain tissue of AD patients, suggesting that EFhd1 might play an important role in the development of AD, while EFhd2 increased in microglia in AD model mice, indicating that EFhd2 might be involved in AD related microglia activation and neuroinflammation.

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Objective To explore the effects of the nerve growth factor neutralizing antibody(anti-NGF) and γ-secretase inhibitor 3,5-difluorophenylacetyl-L-alanyl-S-ph-enylglycine-t-butyl ester(DAPT) on the nuclear expression of p75 neurotrophin receptor(p75NTR) in esophageal cancer Eca109 cells and on cell proliferation and invasion. Methods Immunofluorescence cytochemistry was used to detect the expression changes of p75NTRand Ki67 in Eca109 cells before and after treatment with the anti-NGF and γ-secretase inhibitor DAPT; CCK-8 kit and Transwell cell invasion experiment were used to detect the changes of cell proliferation and invasion ability before and after treatment of Eca109 cells with the anti-NGF and γ-secretase inhibitor DAPT alone or in combination. Results Immunofluorescence cytochemistry showed that after treatment of cells with the anti-NGF and γ-secretase inhibitor DAPT, the number of cells expressing p75NTRin the nucleus decreased, and after the combined treatment of cells with the anti-NGF and γ-secretase inhibitor DAPT, the number of cells expressing p75NTRin the nucleus was the least, and the difference was statistically significant(P<0.05); CCK-8 method and Transwell cell invasion experiment showed that after treatment of cells with the anti-NGF and γ-secretase inhibitor DAPT, cell proliferation and invasion ability were correspondingly weaker than those before treatment, and the difference was statistically significant(P<0.05). Conclusion The anti-NGF and γ-secretase inhibitor DAPT not only inhibit the nuclear transfer of p75NTR, but also reduce the nuclear expression rate of p75NTRand weaken the cell proliferation and invasion ability accordingly.

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Objective To explore the antibacterial coating of polydimethylsiloxane-chlorhexidine gluconate(PDMS-CHXG) constructed on the smooth titanium surface and antibacterial properties forPorphyromonas gingivalisofCHXG solution with different concentrations.Methods The titanium was polished to 7 000 mesh to mirror shape ofabutment, cleaned and dried, then treated with alkalization. The samples were randomly divided into 5 groups:blank control group(C), test groups: grafted CHXG concentration of 0(T0), 0. 4(T1), 0. 8(T2), 1. 6 mg/ml(T3). The surface structural changes were observed by cold field emission scanning electron microscope(CFESEM), and the component elements of coating were analyzed semi-quantitatively. The antibacterial propertiesof the coating were evaluated by antibacterial zone, live/dead bacteria staining and crystal violet experiments.Results A dense film was formed on the surface of titanium under the CFESEM compared with C group. The contentof Cl element increased with the increase of CHXG concentration. There was no inhibition zone around the samplesin C and T0groups, but it was found in T1, T2and T3groups. Live/dead bacteria staining showed no viable bacteria in the T2and T3groups. The results of crystal violet staining showed that T1, T2and T3groups were statistically different from C and T0groups, but the difference between the groups T2and T3was not statistically significant.Conclusion The antibacterial coating of PDMS-CHXG is constructed successfully. The PDMS-CHXG coating dis-plays an exceptional antibacterial property when the concentration of CHXG reaches 0. 8 mg/ml.

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Objective To investigate the effect of the combination of stem cell leukemia(SCL)recombinant lentivirus and connexin 43(Cx43)recombinant lentivirus on the function of diabetic cystipathy(DCP)in guinea pigs. Methods After 90 healthy guinea pigs were fed normally for 1 week, streptozotocin(STZ)was injected intraperitoneally at 200 mg/kg in a single dose, and random blood glucose was monitored weekly. After 12 weeks of normal feeding, 40 guinea pigs meeting the criteria were screened by urodynamic examination and randomly divided into 4 groups: diabetic group, SCL group, Cx43 group, and SCL+Cx43 group. In the diabetic group, 0.2 ml of empty lentivirus without gene was instilled into the bladder through the urethra, in the SCL and Cx43 groups, 0.2 ml of SCL lentivirus and Cx43 lentivirus were instilled using the same method, in the SCL+Cx43 lentivirus group, 0.2 ml of each SCL and Cx43 lentivirus was instilled through the urethra. After 14 days of transfection, urodynamic examination was performed, and then the guinea pigs were executed after the examination, and the bladders were quickly removed for frozen bladder sections and fluorescent double staining. Results There were no differences in urodynamic examinations between the SCL and Cx43 groups compared to the diabetic group(P>0.05). Urodynamic examination in the SCL+Cx43 group showed improvement in detrusor pressure, abdominal pressure and bladder pressure compared to the diabetic group(P<0.05). In laser confocal experiments, the number of interstitial cells of Cajal(ICC) was reduced in the diabetic group, and the spindle structure and cell protrusions were obviously destroyed and appeared in a state of cell lysis. In the SCL and Cx43 groups, there was an improvement in the spindle structure and cell protrusion of ICC-like cells, and there was no significant change in the number of cells. In the SCL+Cx43 group, there was an increase in the number of ICC-like cells, a significant improvement in the spindle structure and cell protrusion, and the formation of ICC-dimers. Conclusion Transurethral co-infusion of SCL and Cx43 gene recombinant lentivirus can be successfully transfected in guinea pig DCP bladder, which can restore the number and structure of damaged ICC cells and form ICC dimer structure, improve the pressure of diabetic bladder forced urinary muscle, improve the weakness of urination, ventral pressure voiding and other characteristics. It provides a new direction for the treatment of DCP.

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Objective To explore the effects of autophagy activation on propofol-induced apoptosis in hippocampal neurons. Methods Primary hippocampal neurons were extracted and isolated from fetal rats and randomly divided into control group, propofol group, rapamycin group and chloroquine group. 2,3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2 H-tetrazolium-5-carboxanilide(XTT) and flow cytometry were used to detect apoptosis, and Western blot was used to detect the expression of autophagy and apoptosis-related proteins. Then, aged rats were randomly divided into control group, propofol group, rapamycin group and chloroquine group. 100 mg/kg propofol was used for continuous anesthesia for 1 week, during which 50 mg/kg rapamycin and 10 mg/kg chloroquine were treated. Morris water maze was used to detect cognitive function, TUNEL staining was used to detect apoptosis, Golgi staining was used to observe autophagy, and Western blot was used to detect autophagy-related apoptosis-related protein expression. Results In vitro, rapamycin obviously reversed the apoptosis caused by propofol, but chloroquine had no effect. Compared with propofol group, the expression of microtubule-associated protein light chain 3 Ⅱ/microtubule-associated protein light chain 3 I(LC3 Ⅱ/LC3 Ⅰ) and Beclin-1 in the rapamycin group increased, but the expression of apoptotic proteins Cleaved-caspase-3 and Bax decreased.In vivo, compared with propofol group, the water maze escape latency of the rapamycin group reduced. In addition, the number of TUNEL-positive cells and autophagosomes in the rapamycin group decreased. Furthermore, the expression of mammalian target of rapamycin(mTOR) in the rapamycin group decreased, and the expression of LC3 Ⅱ/LC3 Ⅰ and Beclin-1 increased, as well as the expression of Cleaved-caspase-3 and Bax decreased. But chloroquine had no effect on autophagy and apoptosis-related proteins. Conclusion Rapamycin can further activate autophagy by inhibiting the activation of mTOR signal, ameliorate the neuronal apoptosis caused by propofol, which will lead to the improvement of the cognition in rats.

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Objective To investigate the effect of ginsenoside Rg1(GRg1) on human retinal microvascular endothelial cells(HRMECs) glycolysis by regulating pyruvate kinase M2(PKM2) expression. Methods HRMECs were cultured in vitro and divided into normal control(NC) group, high glucose(HG) group, high glucose+ginsenoside Rg1(HG+GRg1) group, high glucose+ginsenoside Rg1+low expression PKM2(HG+GRg1+si-PKM2) group, and high glucose+ginsenoside Rg1+overexpression PKM2(HG+GRg1+OE-PKM2) group. si-PKM2 and OE-PKM2 were transfected into HRMECs cells by cell transfection. The expression of PKM2 mRNA in HRMECs was detected by qRT-PCR. The expression levels of related proteins in HRMECs were detected by West-ern blot. The number of lumen formationin vitrowas observed under an inverted microscope to quantify the angio-genesis ability. Cell culture medium of each group was collected, and glucose intake, lactate production and adeno-sine triphosphate(ATP)content were detected by glucose detection kit, lactate detection kit and ATP detection kit,respectively.Results HG induced HRMECs significantly increased the number of blood vessel formation, glycoly-sis and PKM2 expression, while GRg1 treatment significantly reduced the number of blood vessel formation, glycol-ysis and PKM2 expression; transfection of si-PKM2 assisted the inhibitory effect of GRg1 on glycolysis and angio-genesis while transfection of OE-PKM2 interfered with the function of GRg1.Conclusion GRg1 inhibits angiogen-esis by inhibiting PKM2 to reduce glycolysis of HRMECs.

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Objective To observe the effects of TgCtwh3 and TgCtwh6,Toxoplasma gondiiof Chinese 1 dominant genotype, on the ultrastructure of hippocampal of mice. Methods Six-week-old female C57 BL/6 mice were randomly divided into three groups: control group, TgCtwh3 infection group and TgCtwh6 infection group, with 6 mice in each group. Control group was intraperitoneally injected with sterile PBS. The TgCtwh3 infected group was intraperitoneally injected with 4 000 TgCtwh3Toxoplasma gondiitachyzoites and fed for 6 days. The TgCtwh6 infected group was given 20 TgCtwh6Toxoplasma gondiicysts by gavage and fed for 6 weeks. Then, all the animals were sacrificed, the brains were obtained for preparing electron microscope specimens and observed. Results Compared with the control group, the cells in hippocampal of TgCtwh3 infected group showed nuclear shrinkage and necrosis; cytoplasmic and axon swelling; organelle disappearance; axonotmesis and other pathological changes. Compared with the control group, the cells in hippocampal of TgCtwh6 infected group showed swollen, vacuolated, crista-broken or membrane-broken mitochondria; lipofuscin deposition; dilation of golgi apparatus and other pathological changes. Conclusion Both TgCtwh3 and TgCtwh6 infected mice showed ultrastructural changes in hippocampal compared with normal mice. The TgCtwh3 group showed irreversible cell necrosis, while the TgCtwh6 group showed chronic cell damage mainly caused by mitochondrial structure destruction. These results lay a foundation to explore the pathogenesis of toxoplasmosis.

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Objective To analyze the induction effect of dendritic cells(DC)-killer cells(CIK) on cervical cancer cell dormancy. Methods Cervical cancer SiHa cells were selected, the venous blood of cervical cancer patients with cervical cancer was collected and peripheral blood mononuclear cell layer was obtained from density gradient centrifugation, DC cells and CIK cells were induced respectively, the effects of DC-CIK cells on cervical cancer SiHa cells survival rate and proliferation, apoptosis, invasion, migration and other biological behavior were observed after group culture, and Western blot method was used for apoptosis and invasive protein expression. Results Compared with cervical cancer group and DC group, CIK group and DC-CIK group had lower proliferation rate and higher apoptosis rate. Compared with CIK group, the proliferation rate of DC-CIK group was lower and the apoptosis rate was higher(P<0.05). Compared with cervical cancer group and DC group, the survival rate of CIK group and DC-CIK group was lower, and the survival rate of DC-CIK group was lower than CIK group(P<0.05). Compared with cervical cancer group and DC group, the number of invading and migrating cells in CIK group and DC-CIK group was lower. Compared with CIK group, the number of invading and migrating cells in DC-CIK group was lower(P<0.05). Compared with cervical cancer group and DC group, the relative expression levels of phosphatidylinositol 3-kinase(PI3 K), protein kinase B(AKT), mammalian target of sirolimus(mTOR), matrix metalloproteinase-9(MMP-9) in CIK group and DC-CIK group were lower, and the relative expression levels of epithelial calcium adhesin(E-cadherin) were higher. Compared with CIK group, the relative expression levels of PI3 K, Akt, mTOR and MMP-9 were lower in DC-CIK group, and the relative expression levels of E-cadherin were higher(P<0.05). Conclusion The establishment of DC-CIK co-culture system and the intervention of cervical cancer cells can inhibit cervical cancer cell proliferation, invasion and migration, and promote cervical cancer cell apoptosis, thus inducing cervical cancer cell dormancy. The mechanism may be related to the use of DC-CIK cell culture to regulate PI3 K/Akt/mTOR pathway protein and invasion-related protein expression.

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Objective To study the guidance effects of fibroblast growth factor 3(FGF3) on the prethalamic γ-aminobutyric acid(GABA) inhibitory axons, and to explore the effect of FGF3 on the formation of thalamic axonal network. Methods Expression and localization of FGF3 and prethalamic GABA marker gene in chicken embryonic diencephalon were detected by immunofluorescence. Early orientation of GABA axons was tracked by DiI. Three-dimensional gel co-culture experiment was carried out to investigate guidance effects of FGF3 on early prethalamic GABA inhibitory axons.Results Prethalamic GABA cells were adjacent to the FGF3 + hypothalamas, located a-bove the hypothalamus. DiI tracing experiments revealed that prethalamic GABA inhibitory axons had already ex-tended into the thalamus at E6. Compared with the blank control group, FGF3 not only significantly promoted thegrowth of prethalamic GABA inhibitory nerve fibers in the prethalamus, but also repelled the newborn prethalamicaxons to the dorsal thalamus. The number of prethalamic axons was significantly less in proximal section(towardsFGF3 beads) than that in distal section(away from FGF3 beads)(P<0. 01). Moreover, the guidance effects ofFGF3 on prethalamic axons could be blocked by the FGF pathway inhibitor SU5402.Conclusion FGF3, an axonguidance molecule expressed in hypothalamus, exerts guidance effects on the pathway selection of adjacent prethal-amic GABA inhibitory nerve fibers.

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Objective To investigate the effect of long non-coding RNA(LncRNA) anoctamin 1 antisense RNA-1(ANO1-AS1) on the proliferation and apoptosis of esophageal squamous cell carcinoma(ESCC) cells and its possible mechanisms. Methods Silenced ANO1-AS1 lentivirus was transfected in ESCC cells TE-1 and EC109. Subsequently, the expression levels of ANO1-AS1 and calcium-activated chloride channel protein 1(ANO1) in the cells were detected by qRT-PCR. CCK-8 and colony formation assays were used to detect the proliferation of TE-1 and EC109 cells. ANO1 positively related expressed genes were obtained from the LinkedOmics database and then the gene set was enriched for pathways and possible pathways were validated. The expression levels of proliferating cell nuclear antigen(PCNA), P53 protein, apoptosis-related protein(Bax and Bcl-2), ANO1 protein and phosphatidylinositol-3-kinase/protein kinase B(PI3 K/Akt) pathway-related protein were assessed by Western blot. Results After transfection of lentivirus with silent expression function, the expression level of ANO1-AS1 was significantly reduced in TE-1 and EC109 cells(P<0.05); After down-regulation of ANO1-AS1, compared with the negative control group, the proliferation ability of ESCC cells was reduced(P<0.05) and the rate of clone formation decreased(P<0.05); Western blot results showed that, compared with negative controls, the expression of PCNA decreased, the expression of oncogene P53 protein increased(P<0.05), the expression of proteins(Bax) increased, Bcl-2 decreased and the levels of phosphorylation of the pathway proteins PI3 K and Akt decreased(P<0.05). Conclusion Knockdown of ANO1-AS1 can decrease proliferation and promote apoptosis in ESCC, which may be achieved by affecting PI3 K/Akt pathway activation.

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Objective To investigate the effect of hypoxic preconditioning(HPC) on mitochondrial energy metabolism in mouse hippocampal HT22 cells and its possible mechanism. Methods In this paper, mouse hippocampal nerve cells HT22 were divided into control group, hypoxia group, HPC group, and the levels of adenosine triphosphate(ATP) and reactive oxygen species(ROS) in each group were measured for observing the effect of HPC on cell mitochondrial metabolism. Western blot was used to detect the expression of target of rapamycin(mTOR), phosphorylated mTOR protein and autophagy substrate P62 protein; cellular immunofluorescence was used to detect phosphorylated mTOR, and LysoTrackerTMprobe was used to detect lysosomes. Results Compared with the control group, the ATP level was significantly decreased and the ROS level was increased in the hypoxia group. Exposed to HPC, the ATP level was increased and the ROS level was decreased. Compared with the control group, the expression of phosphorylated mTOR was down-regulated and the expression of autophagy substrate P62 was down-regulated in the HPC group. Conclusion HPC may affect the energy metabolism of HT22 cells through the mTOR/autophagy signaling pathway, thereby exerting a protective effect on the HT22 cells.

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Objective To investigate the effects of actin like 6 A(ACTL6 A) knockdown on the proliferation, apoptosis, migration and invasion of SW1990 cells in pancreatic cancer. Methods The Oncomine database was used to analyze the expression of ACTL6 A mRNA in the tissues of pancreatic cancer and normal pancreas. The plasmid of knockdown ACTL6 A and siRNA negative control were established and transfected into SW1990 cell line as siRNA-ACTL6 A group and siRNA-NC group. CCK-8, cell apoptosis experiment, Wound healing and Transwell assay were used to determine the effects of ACTL6 A knockdown on the proliferation, apoptosis, migration and invasion of SW1990 cells. GSEA predicted a possible pathway regulated by ACTL6 A in pancreatic cancer. T-test was used between the two groups. Results The expression of ACTL6 A in pancreatic cancer tissues was higher than that in normal pancreatic tissues(P<0.05). The results of CCK-8 assay showed that the absorbance of siRNA-ACTL6 A group at 24 and 48 h were lower than those in the siRNA-NC group, and the difference was statistically significant(t=5.840, 8.454,P<0.01). The results of Wound healing assay and Transwell assay showed that the healing rate and the number of invasive cells in siRNA-ACTL6 A group were both lower than those in the siRNA-NC group. The difference was statistically significant(t=3.960,4.464,P<0.05), but the apoptosis rate of siRNA-ACTL6 A group was significantly higher than that of the siRNA-NC group, and the difference was statistically significant(t=12.192,P<0.001). GSEA results showed that the group with high expression of ACTL6 A mRNA was up-regulated in cell cycle, nucleotide excision repair, base excision repair, DNA replication, pathways in cancer, NOTCH signaling pathway and other related gene sets(P<0.05). These pathways were activated when the expression of ACTL6 A was up-regulated. Conclusion ACTL6 A is highly expressed in pancreatic cancer tissues. ACTL6 A knockdown promotes the cell apoptosis of SW1990 cells, and inhibits proliferation, invasion and migration of SW1990 cells. The mechanism of the occurrence and development of ACTL6 A in pancreatic cancer is attributed to the activation of cell cycle, nucleotide excision repair, base excision repair, DNA replication, pathways in cancer, NOTCH signaling pathway.

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Objective To investigate whether dual-receptor chimeric antigen receptor T(CART) cells targeting claudin-18 isoform 2(CLDN18.2) and human epidermal growth factor receptor 2(HER2)can reduce the off-target effect and inhibit the growth of gastric cancer cells. Methods Western blot and flow cytometry were used to detect the expression of CLDN18.2 and HER2 in tumor cells. Tumor cell lines overexpressing CLDN18.2 and HER2 were constructed by lentivirus infection. CART cells were constructed by lentivirus infection. Flow cytometry was used to detect the positive rate and subtype distribution of CART cells. Lactic dehydrogenase(LDH) assay was used to detect the cytotoxicity of CART cells against target tumor cells. The tumor suppressive efficiency of CART cellsin vivowas measured using nude mouse xenograft model. Immunohistochemistry was used to detect the infiltration of CD3+T cells in tumor tissues. Results Gastric cancer cell lines with high or no expression of CLDN18.2 and HER2 were successfully screened, and gastric cancer cell lines with single or simultaneous expression of CLDN18.2 and HER2 were constructed. CART cells Cz, Hbb and CHbbz were successfully constructed. Both Cz and CHbbz had specific dose-dependent cytotoxicity against CLDN18.2+and CLDN18.2+HER2+tumor cell linesin vitro(P<0.001). In the nude mouse transplanted tumor model, only CHbbz could produce significant tumor suppressor activity and T cell tumor infiltration against CH-AGS tumor cells(P<0.001), while only CHbbz could produce significant tumor suppressor activity and T cell tumor infiltration against CLDN18.2+HER2+tumor cells(P<0.001). Conclusion Dual receptor CART cells targeting CLDN18.2 and HER2 can reduce the probability of off-target toxicity and inhibit the growth of gastric cancer, which is a potential therapeutic strategy for gastric cancer.

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Objective To investigate the expression of macrophage migration inhibitory factor(MIF) and its effect on autophagy and insulin resistance of ovarian granulosa cells in polycystic ovary syndrome(PCOS). Methods Follicular fluid and ovarian granulosa cells were collected from 40 PCOS patients(n=40) and non PCOS patients(control group, n=20). PCOS included patients with IR(PCOS with IR, n=20) and patients without IR(PCOS without IR, n=20). The expression of MIF in follicular fluid was detected by ELISA. The ratio of autophagy vacuoles to microtubule-associated protein light chain Ⅱ(LC3Ⅱ)/microtubule-associated protein light chain Ⅰ(LC3Ⅰ)in granulosa cells was observed by transmission electron microscopy and Western blot. Human ovarian granule cell line KNG was cultured in vitro and CCK-8 was used to detect the effects of different concentrations of MIF on granule cell activity. KNG cells were divided into four groups: normal culture group(NC group), MIF group, chloroquine group(CQ group) and MIF+CQ group. The effects of MIF on the expression of autophagy related proteins LC3, autophagy related genes 7(Atg7), ubiquitin binding protein(p62), and insulin signaling pathway related proteins insulin receptor substrate-1(ISR-1), glucose transporter 4(GLUT4), protein kinase B(Akt) phosphorylation were observed by Western blot, and the effect of MIF on glucose uptake ability of granulosa cells was detected by glucose uptake test. Results Compared with the control group or PCOS without IR group, MIF expression in follicular fluid and autophagy level of granulosa cells in PCOS with IR group increased(P<0.05 or P<0.01); In vitro experiments showed that MIF could significantly inhibit the cellular activity of KNG in granulocytes in a concentration-dependent manner, in which 100 ng/ml MIF was selected for subsequent relevant experiments; Compared with NC group, LC3Ⅱ/LC3 I ratio and Atg7 protein in MIF group and MIF+CQ group increased(P<0. 05), while p62 protein, IRS-1 protein, Akt phosphorylation level, GLUT4 protein expression level and glucoseuptake ability decreased(P<0. 05), while the above autophagy markers in MIF + CQ group were significantlyhigher than those in MIF group(P<0. 05), and the protein related to insulin signal transduction and glucose up-take increased(P<0. 05).Conclusion MIF may promote IR development in PCOS patients by up-regulating theautophagy level of granulosa cells, while inhibiting the autophagy of granulosa cells can improve MIF-induced IR.

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Objective To investigate the protective effect of obeticholic acid(OCA) on di(2-ethylhexyl) phthalate(DEHP)-induced cholestasis in mice. Methods Animal experiment 1: Female ICR mice were randomly divided into 3 groups: the control group, DEHP low-dose group [50 mg/(kg·d)]and DEHP high-dose group [200 mg/(kg·d)]. All mice were administered with DEHP by gavage for 18 days. Animal experiment 2: Female ICR mice were randomly divided into 4 groups: the control group, OCA group, DEHP model group[200 mg/(kg·d)]and DEHP+OCA group. All mice were administered with DEHP by gavage for 18 days and the duration of OCA was 12-18 days. Serum and liver tissues of mice were collected. Serum alanine aminotransferase(ALT), aspartate aminotransferase(AST), total bile acid(TBA) levels, liver TBA levels, protein expression of farnesoid X receptor(FXR) and mRNA levels of FXR and SHP were detected. HE staining was used to observe the pathological changes in liver tissues. Results Experiment 1: Compared with the control group, the liver weight, liver coefficient and the TBA concentrations in serum and liver significantly increased only in DEHP[200 mg/(kg·d)] group(P<0.01), indicating that the modeling was successful. Animal experiment 2: Compared with the DEHP model group, the liver weight and liver coefficient significantly decreased after OCA treatment, and the TBA concentrations in serum and liver both decreased(P<0.01). Compared with the control group, the protein expression level and its mRNA level of FXR decreased after DEHP[200 mg/(kg·d)]treatment; Compared with the DEHP model group, the protein expression of FXR and the mRNA levels of FXR and SHP significantly increased after OCA treatment(P<0.05). Conclusion DEHP exposure can induce cholestatic liver injury in mice, and OCA posttreatment has a protective effect on DEHP-induced cholestasis in mice.

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Objective To investigate the effects of glycated low density lipoprotein(Gly-LDL) on the growth of human umbilical vein endothelial cells(HUVECs) and the expression of toll like receptor 4(TLR4) and hypoxia inducible factor-1α(HIF-1α), and to explore its possible mechanism. Methods HUVECs were culturedin vitroand divided into control group, positive control group[50 mg/L normal low density lipoprotein(n-LDL)], low concentration, medium concentration and high concentration Gly-LDL(50, 75, 100 mg/L) groups. Respectively, the effects of different concentrations of Gly-LDL on survival rate of HUVECs were detected by CCK-8; The motility of HUVECs under different treatments were detected by wound healing assays; The level of inflammatory cytokine, such as tumor inducing factor-α(TNF-α), interleukin-6(IL-6), intercellular adhesion molecule-1(ICAM-1) and vascular cell adhesion molecule-1(VCAM-1) were detected by ELISA; The mRNA levels of TLR4, HIF-1α, TNF-α and IL-6 were detected by qRT-PCR; Protein expressions of TLR4, HIF-1α, TNF-α and IL-6 were detected by Western blot; Respectively, si-RNA of TLR4 and HIF-1α was used to intervene the effects of Gly-LDL on HUVECs. The experiment was divided into control group, model group(Gly-LDL 100 mg/L), si-TLR4 group(Gly-LDL 100 mg/L+si-TLR4), TLR4 unloading group(Gly-LDL 100 mg/L+si-NC1), si-HIF-1α group(Gly-LDL 100 mg/L+si-HIF-1α) and HIF-1α unloading group(Gly-LDL 100 mg/L+si-NC2). Protein expressions of TLR4 and HIF-1α were detected by Western blot to verify the interaction between TLR4 and HIF-1α. Results The survival rate and migration rate of HUVECs were inhibited in Gly-LDL(50 mg/L, 75 mg/L, 100 mg/L) group(P<0.01), the inflammatory cytokines, such as TNF-α, IL-6, ICAM-1,VCAM-1 increased by Gly-LDL function on HUVECs(P<0.001), and the mRNA and protein levels of TLR4, HIF-1α, TNF-α and IL-6 increased by Gly-LDL in a dose dependent manner. After TLR4 was knocked out, the proteins expression of TLR4 and HIF-1α were down-regulated compared with model group(P<0.05),but after HIF-1α was knockout, only the protein expression of HIF-1α was down-regulated compared with model group(P<0.01),while the protein expression of TLR4 was up-regulated under the influence of Gly-LDL. Conclusion Gly-LDL may inhibit the proliferation and migration of HUVECs by up-regulating TLR4/HIF-1α inflammatory signaling pathway, and promote the expression of inflammatory cytokines, leading to vascular endothelial injury.

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Objective To explore the effect of LncRNA MEG3 in temozolomide(TMZ) resistance of glioma cells. Methods TMZ-resistant glioma cells U87(U87/TMZ) were constructed, qRT-PCR was used to detect the LncRNA MEG3 expression level, and MTT was used to detect the cell proliferation ability. The LncRNA MEG3 in U87/TMZ was overexpressed by liposome method(pcDNA-MEG3 group), the empty vector was transfected as the empty vector group(pcDNA group), and the conventionally cultured U87/TMZ was used as the blank group(Control group),then treated with 10 μg/ml TMZ. The plasmid transfection effect was analyzed by qRT-PCR. The expression of LncRNA MEG3-related protein was confirmed by Western blot. The cell cloning experiment detects the effect of LncRNA MEG3 on cell proliferation. The effect of LncRNA MEG3 on cell invasion was tested by Transwell. The effect of LncRNA MEG3 on cell apoptosis was detected by flow cytometry. And mouse transplant tumor animal model was constructed forin vivoexperiments. Results Compared with U87, the expression level of LncRNA MEG3 in U87/TMZ was lower(P<0.01), and the cell viability of U87/TMZ was higher than that of U87(P<0.01). After overexpression of MEG3 combined with TMZ, compared with the Control group and the pcDNA group, the pcDNA-MEG3 group up-regulated the expression of MEG3 mRNA and p53 protein, and down-regulated the expression of MDM2 protein, and the proliferation and invasion ability of the pcDNA-MEG3 group decreased while the apoptosis ability increased(P<0.01). The construction of mouse transplant tumor animal model showed that the tumor volume and mass of the pcDNA-MEG3 group were reduced compared with the pcDNA group(P<0.01). Conclusion Overexpression of LncRNA MEG3 can attenuate the drug resistance of TMZ-resistant glioma cells.

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Objective To explore the killing effect of chimeric antigen receptor NK-92(CAR-NK-92) cells targeting mesothelin(MSLN) on ovarian cancer cells. Methods The expression of MSLN in primary ovarian cancer tissues and ovarian cancer cell lines was detected by immunohistochemical analysis and immunofluorescence, respectively. The CAR-NK-92 cells targeting MSLN were constructed by lentiviral transfection, the transfection efficiency was detected by flow cytometry, and the killing effect of CAR-NK-92 on ovarian cancer was verifiedin vivoandin vitro. Results MSLN was highly expressed in primary ovarian cancer tissues and ovarian cancer cell lines. Flow cytometry showed that the purity of CAR-NK-92 targeting MSLN was about 70%. The experiments found that MSLN-CAR-NK-92 cells had a strong anti-ovarian cancer effectin vivoandin vitro, and could release more cytokines interferon-γ and tumor necrosis factor-α. Conclusion MSLN-CAR-NK-92 has strong anti-ovarian cancer effectsin vivoandin vitro, providing a new potential treatment option for ovarian cancer patients.

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Objective To investigate the protective effect and related mechanisms of dexmedetomidine(Dex) on iron overload toxicity in mouse hippocampal neurons(HT22) induced by ferric ammonium citrate(FAC). Methods Selected HT22 cells in good condition were randomly divided into 4 groups: control group(Ctrl group), FAC treatment group(FAC group), Dex treatment group(Dex group), ferroptosis inhibitor Fer-1 treatment group(Fer-1 group). The iron overload model was established by FAC-induced cells. Subsequently, the proliferation and survival rate of HT22 cells was detected by CCK-8 method; Western blot was used to detect the ferroptosis marker proteins prostaglandin-endoperoxide synthase 2(PTGS2) and acyl-CoA synthetase long-chain family member 4(ACSL4). The protein expressions of mammalian target of rapamycin(mTOR), transferrin receptor 1(TFR1) and ferroportin(Fpn); the gene expression levels of PTGS2 and ACSL4 in HT22 cells were detected by qPCR; Reactive oxygen species(ROS) levels in HT22 cells was detected by DHE fluorescent probe; MDA detection kit was used to detect lipid oxidation levels in HT22 cells; Mito-FerroOrange—ferrous ion probe was used to detect ferrous ion levels in HT22 cells; electron microscopy was used to detect intracellular ultrastructural changes. Results Dex group and Fer-1 group significantly decreased cell death rate after 2 h of pretreatment, the protein and gene expression levels of ferroptosis markers PTGS2 and ACSL4 significantly decreased. The degree of cell ultrastructural damage was significantly improved. The levels of ROS, lipid oxidation and Fe2+were significantly lower than those of the FAC group(P<0.05). Conclusion Dex pretreatment can attenuate FAC-induced iron overload toxicity injury in HT22 cells, which may be related to the inhibition of ferroptosis.

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Objective To investigate the effects of fukutin(FKTN) which is a member of ribitol-5-phosphate transferases on HeLa cell cycle, apoptosis, migration and the secretion of α-dystroglycan(α-DG). Methods The plasmid pcDNA3.1-α-DAG-HA was constructed. HeLa cells were transfected with plasmid pcDNA3.1-FKTN-3 xFlag, then the total protein was extracted for Western blot to determine the expression level of FKTN. Cell cycle and apoptosis were measured by flow cytometry after the overexpression of FKTN. Following the overexpression of FKTN, wound-healing assay was performed to detect the cell migration rate, as the same time wheat germ agglutinin-agarose(WGA-agarose) was used to enrich α-DG in the cell cultures, then α-DG was detected by Western blot.Results The eukaryotic expression plasmid pcDNA3.1-α-DAG-HA was constructed successfully. FKTN could be overexpressed in HeLa cells. After the overexpression of FKTN,the percentage of S phase of cell cycle in the experimental group decreased(P<0.001) and apoptosis rate unchanged(P>0.05) when compared with the control group. There was no change in the cell migration rate of experimental group(P>0.05), but after the overexpression of FKTN, secretion of α-DG decreased when compared with control group.Conclusion Overex-pressing FKTN arrests cell cycle and inhibits the secretion of α-DG in HeLa cells. Apoptosis and cell migration ofHeLa cells are not affected by the overexpression of FKTN.

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Objective To study the relationship between the expression of eIF4F complex and human colorectal cancer (CRC),and the possible mechanism of sulforaphane on the proliferation of CRC cells.Methods Immunohistochemical staining was used to detect the expression of eIF4F complex related proteins in tumor and adjacent tissues of 12 colorectal cancer patients.MTT colorimetry was used to detect the cell activity of colorectal cancer cells treated with sulforaphane.Western blot was used to detect the expression of eIF4F complex related proteins in colorectal cancer cells treated with sulforaphane.In the animal experiment (tumor formation in nude mice),the growth of subcutaneous tumors in nude mice after intraperitoneal injection of sulforaphane was compared,and the expressions of PI3K/AKT/mTOR/4EBP1 signal pathway related proteins and eIF4F complex related proteins were detected by immunohistochemical staining.Results In the specimens of CRC patients,the expression of eIF4F complex related protein in tumor tissues was significantly higher than that in paracancerous tissues.SFN could inhibit the proliferation of CRC cells,and the higher the concentration,the stronger the inhibitory ability.SFN could inhibit the growth and development of CRC cells and down-regulate the expression of eIF4F complex in CRC cells.Conclusion The expression of eIF4F complex is closely related to the development of colorectal cancer.Sulforaphane may affect the up-regulation of eIF4F complex through PI3K/AKT/mTOR/4EBP1 signal pathway,thus affecting the development of colorectal cancer cells.

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Objective To explore the relationship between the levels of plasma lipoprotein-associated phospholipase A2(LP-PLA2), lipoprotein(a) [LP(a)] and the SYNTAX score in patients with coronary heart disease(CHD). Methods A total of 416 patients diagnosed with CHD by coronary angiography(CAG) were enrolled. According to the SYNTAX score, they were divided into three groups, 364 cases in the low-risk group(score of 0-22), 39 cases in the medium-risk group(score of 23-32), and 13 cases in the high-risk group(score of 33 or more). And another 30 cases which CAG showed no significant narrowing of the coronary arteries were selected as the control group(CON), We detected and compared the differences of the levels of LP-PLA2 and LP(a) in various groups. According to the levels of LP-PLA2 and LP(a), CHD patients were divided into four groups, 225 cases in the LP-PLA2 and LP(a) normal group, 35 cases in the only LP(a) elevated group(≥300 mg/L), 43 cases in the only LP-PLA2 elevated group(≥175 ng/ml) and 113 cases in the LP-PLA2 and LP(a) elevated group. The differences of the SYNTAX score, stenosis degree score and lesion feature score were compared. Pearson correlation and multiple linear regression were used to analyze the correlation between the levels of LP-PLA2, LP(a) and the SYNTAX score, stenosis degree score and lesion feature score. Results The levels of LP-PLA2 and LP(a) in the CHD group were significantly higher than those in the CON group(P<0.05). The levels of LP-PLA2 and LP(a) in the subgroups according to the SYNTAX score were declining from the high-risk group to the low-risk group in decending order(P<0.05). The SYNTAX score and stenosis degree score of the LP-PLA2 and LP(a) elevated group were significantly higher than those of the only LP-PLA2 elevated group, the only LP(a) elevated group, the LP-PLA2 and LP(a) normal group(P<0.05), but there was no significant difference in lesion feature score among the groups(P>0.05). Pearson correlation analysis showed that the SYNTAX score, stenosis degree score and lesion feature score were positively correlated with the levels of LP-PLA2 and LP(a)(P<0.05). The multiple linear regression showed that the SYNTAX score and stenosis degree score were independently correlated with the levels of LP-PLA2 and LP(a) after adjusting for confounding effects(P<0.05), while lesion feature score was only independently correlated with the levels of LP-PLA2(P<0.05). Conclusion The levels of LP-PLA2 and LP(a) show a positive correlation with the SYNTAX score in patients with CHD, and both can be used as indicators to determine the severity of coronary artery disease.

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Objective This study aims to explore the general rules of profile-based forehead protrusion value and appearance in Anhui province, and the results will provide guidance for clinical orthodontic works on matching lateral forehead protrusion and the lower third of the face in the future. Methods A total of 262 patients were included in this cross-sectional study. The materials for the study were the lateral cephalometric radiographs and standard 90-degree profile photographs taken from the subjects at the same time. The Frankfurt horizontal(FH) plane was used to calibrate the head position parallel to the ground. The samples were grouped according to genders, ages and dentoskeletal classifications. The measurement points of forehead protrusion in profile included hairline point and soft tissue nasion point. The point where the parallel line connecting two points was tangent to the forehead contour was defined as the most convex point. The value of forehead protrusion in profile was expressed by the distance between two parallel lines. The forehead morphology was studied by tracing the forehead on lateral photographs.Pvalues<0.05 were considered statistically significant. Results The lateral forehead protrusion of female was larger than that of male, and the difference between male and female decreased gradually from children(P<0.001), adolescents(P<0.001) to adults. The shape of the forehead differed between the two sexes. Female forehead is round, protruding part in the middle, the overall appearance like an arc; Male forehead was more straight, the most convex point was generally located near the eyebrow arch. Conclusion Gender is an important factor influencing profile forehead protrusion and appearance. The difference between male and female forehead protrusion decreases with age. But the shape remains different.

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Objective To investigate the relationship between the 24 h ambulatory blood pressure circadian rhythm abnormalities and kidney damage in the patients of lupus nephritis(LN). Methods A total of 103 patients with LN patients were enrolled retrospectively. All patients were accepted 24 h ambulatory blood pressure monitoring(ABPM). The patients were divided into 2 groups according to the 24 h ambulatory blood pressure circadian rhythm, including nocturnal blood pressure meaning average declining during the day(>10%) and non-dipper type blood pressure group(<10%). The kidney damage index of LN patients with or without hypertension or noc-turnal blood pressure and non-dipper type blood pressure was analyzed. The influencing factors of the circadianrhythm of LN blood pressure were analyzed by binary Logistic regression.Results Among the 103 LN patients, 66patients were hypertension, 37 patients were none hypertension. Fifty-nine patients were non-dipper type bloodpressure in LN with hypertension group, and 30 patients were non-dipper type blood pressure in LN without hyper-tension. There was no significant difference in the frequency of non-dipper type blood pressure between the twogroups(81. 1%vs89. 4%, χ2= 1. 395,P= 0. 238). Compared with hypertension group, the levels of serum cre-atinine(Z= 2. 911,P= 0. 004), urea(Z= 3. 348,P= 0. 001) and uric acid levels(t= 2. 017,P= 0. 047)were significantly higher than those of LN without hypertension patients, whereas the levels of glomerular filtrationrate(eGFR)(Z= 4. 846,P<0. 001) were significantly lower than those of LN without hypertension patients. Inthe group of LN with hypertension, the levels of uric acid(Z= 2. 893,P= 0. 004) were significantly higher thanthose of nocturnal blood pressure subgroup patients compared with no dipper type blood pressure subgroup patients,and the levels of eGFR(Z= 2. 017,P= 0. 0440) were significantly lower. Nevertheless, in the group of LN with-out hypertension, the kidney damage index had no significant difference between the two subgroups. Univariate andmultivariate analysis showed that uric acid was associated with an abnormal rhythm of LN combined with hyperten-sion.Conclusion Abnormal blood pressure rhythms are common in LN patients with or without hypertension. Re-nal damage significantly increases in the non-dipper group of LN compared with hypertension patients.

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Objective To analyze the length of hospital stay of patients with heart failure in a multicenter cohort in order to explore the influencing factors of length of stay and provide data support for further intervention. Methods A total of 2 794 patients enrolled in the multicenter prospective heart failure cohort were divided into two groups: long hospital stay group(≥9 days) and short hospital stay group(<9 days). The general data of the two groups were compared, and the factors with statistical difference in univariate analysis were included in Logistic multifactor regression analysis to explore the difference in length of hospital stay between the two groups. According to left ventricular ejection fraction(LVEF), patients were divided into heart failure with preserved reduced ejection fraction(HFpEF)group, heart failure with mildly reduced ejection fraction(HFmrEF)group and heart failure with reduced ejection fraction(HFrEF) group, and Logistic multifactor regression analysis was performed to find influencing factors. Results Logistic multifactor regression analysis showed that LVEF, pneumonia, N-terminal pro-B-type natriuretic peptide(NT-proBNP), serum sodium, cardiac resynchronization therapy(CRT)or implantable cardioverter defibrillator(ICD)implantation, β blockers, aldosterone receptor antagonists, positive inotropic drugs and vasodilators were all factors influencing the hospitalization of HF patients. In the HFpEF, HFmrEF, and HFrEF groups, CRT/ICD implantation, positive inotonic drugs, and vasodilator use were suggested to be common factors affecting length of hospital stay in all three groups. Conclusion LVEF, pneumonia, NT-proBNP, serum sodium, CRT or ICD implantation, β blockers, aldosterone receptor antagonists, positive inotropic drugs and vasodilators are the influencing factors of hospitalization time in HF patients.

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Objective To study the correlation between cerebral white matter structure with cognitive function and blood biochemical indexes in patients with end stage renal disease(ESRD). Methods The diffusion tensor imaging(DTI) data of 64 ESRD patients and 47 age and sex matched healthy people were collected. Tract-based spatial statistics(TBSS) and XTRACT analysis methods were used to compare the differences in diffusion parameters between the two groups. Pearson correlation analysis was used to detect the correlation between various diffusion parameters and blood biochemical indexes and cognitive related scales. Results The values of FA in the ESRD group generally decreased(P<0.05). The values of MD, AD and RD obviously increased(P<0.05). The scores of mini mental state examination(MMSE) and Montreal cognitive assessment(MoCA) decreased(P<0.01), while that of trail making test A part(TMT-A) increased(P<0.05). In the ESRD group, the values of FA in the right anterior thalamic radiation, optic radiation, acoustic radiation, and cingulum were negatively correlated with thelevels of creatinine and urea nitrogen, and positively correlated with MoCA′s scores, and the values of MD and RDof these tracts were positively correlated with the concentration of urea. The values of FA in optic radiation, acous-tic radiation and left temporal of cingulum were negatively correlated with the scores of TMT-A. The values of FA inthe vertical occipital fasciculus, inferior fronto-occipital fasciculus, left middle longitudinal fasciculus and forcepsmajor were negatively correlated with the concentration of creatinine. The values of FA in the left arcuate fasciculuswere positively correlated with the MoCA′s score, the values of MD and RD of these tracts were positively correlatedwith the concentration of creatinine, and negatively correlated with the scores of MMSE and MoCA. The values ofFA in the right superior longitudinal fasciculus, the right corticospinal tract and the right frontal aslant tract werepositively correlated with the MoCA′s score, the values of MD and RD of these tracts were positively correlated withthe concentration of urea; the values of MD and RD in the left fornix were positively correlated with the level of cre-atinine and urea.Conclusion The structural integrity of white matter in ESRD patients is extensively damaged,which is significantly associated with a variety of cognitive impairments. Serum creatinine and urea nitrogen may berisk factors for the changes in white matter.

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Objective To explore the effects of surgical treatment for benign ovarian tumors on reproductive outcomes. Methods One hundred and twenty-six patients(case group) who underwentin vitrofertilization and intracytoplasmic sperm injection-embryo transfer(IVF/ICSI-ET) and a history of benign ovarian tumor surgery were retrospectively analyzed. Totally one hundred and forty patients who underwent IVF/ICSI-ET due to female tube factors during the same period were matched as control group. The general characteristics, the first controlled ovarian hyperstimulation(COH) process, clinical outcomes and cumulative reproductive outcomes were then compared. Results There was no significant difference in the general characteristics, total dosage of gonadotropin(Gn) used, duration of Gn used, estrogen(E2) level and intima thickness on human chorionic gonadotropin(HCG) injection day during the first COH period between the two groups(P>0.05). There was no significant difference in the number of oocytes retrieved, number of MII, follicle-oocyte index(FOI) and ovarian sensitivity index(OSI)(P>0.05). However, the number of transferable embryos and high-quality embryos in control group was significantly higher than that in case group(P<0.05), while the transferred embryo number, implantation rate and clinical pregnancy rate were not statistically significant(P>0.05). Moreover, the cumulative pregnancy rate and live birth rate, clinical pregnancy rate and live birth rate per transplant cycle, the number of oocytes retrieval cycles required per live birth, the number of transplant cycles required per live birth, and the number of embryos required per live birth were also not statistically significant between the two groups(P>0.05). Conclusion Compared with control group, the clinical outcomes of assisted reproductive therapy in benign ovarian tumors infertility patients with normal ovarian reserve after surgical treatment are not significantly different.