〔Abstract〕 Objective To investigate the effect of ginsenoside Rg1(GRg1) on human retinal microvascular endothelial cells(HRMECs) glycolysis by regulating pyruvate kinase M2(PKM2) expression. Methods HRMECs were cultured in vitro and divided into normal control(NC) group, high glucose(HG) group, high glucose+ginsenoside Rg1(HG+GRg1) group, high glucose+ginsenoside Rg1+low expression PKM2(HG+GRg1+si-PKM2) group, and high glucose+ginsenoside Rg1+overexpression PKM2(HG+GRg1+OE-PKM2) group. si-PKM2 and OE-PKM2 were transfected into HRMECs cells by cell transfection. The expression of PKM2 mRNA in HRMECs was detected by qRT-PCR. The expression levels of related proteins in HRMECs were detected by West-ern blot. The number of lumen formationin vitrowas observed under an inverted microscope to quantify the angio-genesis ability. Cell culture medium of each group was collected, and glucose intake, lactate production and adeno-sine triphosphate(ATP)content were detected by glucose detection kit, lactate detection kit and ATP detection kit,respectively.Results HG induced HRMECs significantly increased the number of blood vessel formation, glycoly-sis and PKM2 expression, while GRg1 treatment significantly reduced the number of blood vessel formation, glycol-ysis and PKM2 expression; transfection of si-PKM2 assisted the inhibitory effect of GRg1 on glycolysis and angio-genesis while transfection of OE-PKM2 interfered with the function of GRg1.Conclusion GRg1 inhibits angiogen-esis by inhibiting PKM2 to reduce glycolysis of HRMECs.