〔Abstract〕 Objective To clarify the impact of GATA2 overexpression on transcriptional expression in chicken preadipocytes. Methods Chicken preadipocytes ICP1 were cultivated, pCMV-myc-GATA2 or pCMV-myc plasmid was transfected, Western blot was used to verify the overexpression of GATA2 in cells, RNA-seq was used to study the changes in cell transcriptome expression caused by overexpression of GATA2,ChIP-seq was used to study the binding of GATA2 in the genome, Real time PCR was used to verify some differentially expressed genes, ChIP-PCR was used to verify the collection of GATA2 to TAF3 genes. Results Transfection of pCMV-myc-GATA2 allowed overexpression of GATA2 protein in ICP1 cells; overexpression of GATA2 resulted in up-regulation of 942 genes and down-regulation of 840 genes in ICP1 cells(P<0.05),and enrichment analysis showed that differentially expressed genes were associated with ribosome and mitochondrial structure and function(P<0.01);GESA analysis showed that the expression of GATA2-overexpressing ICP1 cells showed up-regulation of RNA polymerase Ⅱ transcription initiation complex, ribosome biogenesis, propionate metabolism, and oxidative phosphorylation-related gene sets, and down-regulation of histone lysine N-methyltransferase, nuclear transcription repression complex formation, adipokines, and calcium ions signaling pathway-related gene sets.ChIP-seq in ICP1 cells identified 2833 GATA2-binding genomic peaks involving 2018 genes.motif analysis revealed GATA2-binding(T/A) GATA motifs; enrichment analysis showed that these genes are involved in embryonic development, signaling, cellular metabolism, and cell-cell interactions.Differently expressed genes and GATA2-binding genes were taken to intersect to obtain 105 genes, and enrichment analysis showed that these genes were associated with transcription, post-transcriptional regulation, cell-cell interactions, signaling, and cell cycle(P<0.001). Conclusion GATA2 can at least bind to the genome of chicken preadipocytes to regulate their transcriptome expression patterns.