〔Abstract〕 Objective To explore the effect of long-chain non-coding RNA(lncRNA) SPINT1-AS1 on the growth and invasion of ovarian cancer cells and the molecular mechanism. Methods The expression of lncRNA SPINT1-AS1 in ovarian cancer tissues and normal tissues was analyzed by GEPIA database.Fluorescence real-time quantitative polymerase chain reaction(qRT-PCR) method was used to determine the expression of lncRNA SPINT1-AS1 in ovarian cancer cell lines SKOV-3,A2780,OC3,HO-8910 and immortalized ovarian epithelial cell line IOSE80.The SKOV-3 cells were divided into si-SPINT1-AS1 group(transfected with lncRNA SPINT1-AS1 siRNA) and si-NC group(transfected with siRNA control).The effect of down-regulated lncRNA SPINT1-AS1 on the proliferation and invasion of SKOV-3 cells was determined by MTT method and Transwell chamber experiment.Western blot was used to detect the expression of proliferative phenotypic proteins(PCNA,CyclinD1) and metastatic phenotypic proteins(MMP2,MMP9).The dual luciferase reporter system was used to identify the targeting relationship between lncRNA SPINT1-AS1 and miR-211-5 p.The effect of down-regulated lncRNA SPINT1-AS1 on the expression of miR-211-5 p was detected by qRT-PCR. Results Compared with normal tissues, the expression level of lncRNA SPINT1-AS1 in ovarian cancer tissues was higher(P<0.01).Compared with IOSE80 cells, the expression level of lncRNA SPINT1-AS1 in ovarian cancer cell lines SKOV-3,A2780,OC3,HO-8910 increased(P<0.05),and the expression level of lncRNA SPINT1-AS1 in SKOV-3 cells was the highest(P<0.01).Compared with the si-NC group, the absorbance of SKOV-3 cells in the si-SPINT1-AS1 group was reduced(P<0.05),the number of invasive cells was reduced(P<0.05),and the expression of proliferation phenotype proteins PCNA and CyclinD1 were reduced(P<0.01),the expression of metastatic phenotypic proteins MMP2 and MMP9 was reduced(P<0.01).lncRNA SPINT1-AS1 could directly bind to miR-211-5 p(P<0.01).Compared with the si-NC group, the expression level of miR-211-5 p in SKOV-3 cells of the si-SPINT1-AS1 group increased(P<0.01). Conclusion The expression of lncRNA SPINT1-AS1 in ovarian cancer tissues and cell lines increased.Down-regulation of lncRNA SPINT1-AS1 can inhibit the proliferation and invasion of ovarian cancer SKOV-3 cells by targeting up-regulation of miR-211-5 p.