〔Abstract〕 To investigate the differences in enolase and laminin levels in vaginal epithelial tissues be- tween mice successfully infected with Gardnerella and mice not infected with Gardnerella , providing information for further exploration of the correlation between enolase and laminin levels and the incidence of bacterial vaginosis . Methods Gardnerella strains isolated , purified , and identified from vaginal secretions of patients with bacterial vaginosis were used to infect the vagina of mice and establish a mouse model of bacterial vaginosis . Successful and failed mice was defined as successful and failed groups , respectively . Differential expression of enolase and laminin in the vaginal epithelial tissue of two groups of mice was detected by Western blot. Modeling success rate was statis- tically analyzed , and the expression differences of enolase and laminin was compared between two groups . Results One strain of Gardnerella vaginalis infected 10 SPF grade KM mice , 7 mice met the diagnostic criteria for bacte- rial vaginosis , and 3 mice failed to model , with a success rate of 70% . Western blot was used to detect protein ex- pression levels , and the levels of laminin and enolase in the successfully modeled mouse vaginal epithelial tissue were significantly higher than those in the failed modeling group , with statistical differences between the two groups (P < 0 . 05) . Conclusion Enolase and laminin may be involved in the occurrence of bacterial vaginosis , however , further research is needed to determine the mechanisms through which they trigger the occurrence and development of the disease .