Found programs: National Natural Science Foundation of China(No.32000906);Natural Science Foundation of Hubei Province(No.2024AFB1024)
Authors：Ao Xin; Long Yunfeng; Zhang Zhengrong; Zhang Mingzhu; Le Zhuang; Su Yanting
Keywords：C1GALT1; glioblastoma; cell proliferation;cell migration; cell invasion; cell senescence;
DOI：10.19405/j.cnki.issn1000-1492.2025.06.003
〔Abstract〕 Objective To explore the expression profile of core 1 β1,3-galactosyltransferase 1(C1GALT1) in glioblastoma(GBM) and to elucidate its impact on the initiation and progression of GBM. Methods The expression levels and prognostic significance of C1GALT1 in GBM were analyzed using the GEPIA and CGGA databases. Two representative glioblastoma cells(U251 and LN18) were selected to construct C1GALT1-knockdown cell lines and performed in vitro experiments. The Cell Counting Kit-8(CCK-8) and Transwell assays were employed to evaluate the impact of C1GALT1 on proliferation, migration and invasion of GBM cells. Transcriptome data were analyzed to identify potential signaling pathways. Senescence β-Galactosidase Staining Kit was used to detect β-galactosidase activity. Results Analysis of GEPIA and CGGA databases revealed that C1GALT1 was significantly upregulated in GBM tissues compared to adjacent non-cancerous tissues(P