Found programs: Natural Science Research Project of Anhui Educational Committee (No. 2022AH051141);Doctoral Scientific Research Fundation of the First Affiliated Hospital of Anhui Medical University (No. BSKY-2019002)
Authors：Xiao Rong; Xue Yan; Gao Xiaoman; Zhang Yaqi; Wang Lin; Deng Datong
Keywords：hypothalamic-pituitary-ovarian axis; acarbose; pioglitazone; rat; sex hormone; ovarian hormone;
DOI：10.19405/j.cnki.issn1000-1492.2025.07.012
〔Abstract〕 Objective To investigate the effects of acarbose and pioglitazone on the levels of sex hormones in adult female rats, and to explore their regulatory effects on the hypothalamic-pituitary-ovarian axis(HPOA) and its mechanism. Methods Adult female SD rats aged 2 months were randomly divided into 0.9% sodium chloride solution control group(NS), acarbose low(AL), medium(AM) and high(AH) dose groups and pioglitazone low(PL), medium(PM) and high(PH) dose groups. AL, AM and AH groups were given 20, 40 and 60 mg/(kg·d) acarbose, and PL, PM and PH groups were given 5, 10 and 15 mg/(kg·d) pioglitazone, respectively. After 28 days of intervention, serum gonadotropin(FSH, LH) and ovarian secreted hormone(E2, P, T) were detected by ELISA, and the effects of acarbose and pioglitazone on HPOA were investigated by organ index analysis and histopathological observation of pituitary gland and ovary. Results Compared with the NS group, the serum FSH in the PL, PM and PH groups decreased(all P