〔Abstract〕 Objective To construct a lentiviral overexpression cell line of HACE1 and to investigate its effects on the proliferation and migration of glioma cells. Methods Using the bacterial liquid containing the full-length cDNA sequence of human HACE1 as a template, the lentiviral expression plasmid pCDH-HACE1 was constructed, and the lentiviral cell line was screened. Western blot was employed to detect the expression of HACE1 in glioma cells. Meanwhile, the CCK-8 assay and the cell scratch assay were utilized to assess the impacts of HACE1 on the proliferation and migration of glioma cells. Results The lentiviral cell line with HACE1 overexpression was successfully established. Western blot analysis demonstrated that HACE1 could be effectively expressed in eukaryotic cells. The results of the CCK-8 assay showed that, compared with the control group, the cell proliferation in the HACE1 overexpression group was significantly enhanced at 24 hours and 48 hours after cultivation(both P