Found programs: Natural Science Research Projects of Anhui Educational Committee (No. KJ2021A0807);Graduate Student Research Innovation Project of Bengbu Medical University (No. Byycxz21087)
Authors：Zhao Zhi; Liu Mengkun; Zha Rifei; Zhang Tingbao; Wang Cheng
Keywords：MALAT1; miR-15b-5p; chondrocyte; Wnt/β-catenin signaling pathway; osteoarthritis; inflammation response; osteogenic differentiation; extracellular matrix;
DOI：10.19405/j.cnki.issn1000-1492.2025.07.010
〔Abstract〕 Objective To explore the molecular mechanism of long non-coding RNA metastasis associated lung adenocarcinoma transcript 1(MALAT1)/microRNA-15b-5p(miR-15b-5p) regulating the Wnt/β-catenin pathway in lipopolysaccharide(LPS)-induced chondrocyte injury in osteoarthritis. Methods ATDC5 cells were treated with 5 mg/L LPS to establish the osteoarthritis cell injury model, and the expression levels of MALAT1 and miR-15b-5p in the cells were detected by RT-qPCR. The MTT, flow cytometry, Alizarin red staining, and ELISA were used to assess the effects of MALAT1 and miR-15b-5p on LPS-induced chondrocyte injury. The dual-luciferase reporter gene assay was used to examine the regulatory relationship between MALAT1 and miR-15b-5p. Western blot assay was used to evaluate the expression of relevant proteins. Results In LPS-induced ATDC5 cells, MALAT1 expression decreased(P