Found programs: Natural Science Foundation of Anhui Province (No. 2108085MH320);Research Project of Anhui Provincial Institute of Translational Medicine (No. 2023zhyx-C11)
Authors：Zong Xuemei; Lin Qianqian; Chen Yuelan; Wang Xinming; Wei Wei; Yan Shangxue; Chang Yan
Keywords：rheumatoid arthritis; kynurenine 3-monooxygenase; kynurenine; 3-hydroxykynurenine;peripheral blood mononuclear cells; synovial tissue; fibroblast-like synoviocytes;
DOI：10.19405/j.cnki.issn1000-1492.2025.07.008
〔Abstract〕 Objective To investigate the expression and clinical significance of kynurenine-3-monooxygenase(KMO) in peripheral blood mononuclear cells(PBMC), synovial tissue, and fibroblastic-like synovial cells(FLS) of rheumatoid arthritis(RA) patients. Methods Peripheral blood samples from 25 healthy control(HC) individuals and 25 patients diagnosed with RA were collected, and real-time fluorescence quantitative PCR and Western blot were used to detect KMO gene and protein expression in PBMC of RA and HC groups, and to analyze the correlation between the expression level of the KMO gene in the PBMC of the RA patients and the indexes of the laboratory tests. Meanwhile, immunohistochemistry and immunofluorescence were used to detect KMO expression in synovial tissue and FLS in RA and HC groups. Results(1) KMO gene and protein expression in PBMC of RA group were higher than that of HC group, and the difference was statistically significant(Ps=0.417, P=0.038; r=0.545, P=0.005; rs=0.433, P=0.031), and had no correlation with C-reactive protein and anti-cyclic citrullinated polypeptide antibody.(3) KMO expression in synovial tissue of RA group was higher than that of HC group, and the difference was statistically significant(P