Found programs: National Natural Science Foundation of China (No. 82001747);Basic and Applied Basic Research Foundation of Guangdong Province (No. 2020A1515110562)
Authors：Li Yutong; Luo Xiaoqi; Tan Qifa; Chen Mingjie; Wu Changyou; Shen Juan
Keywords：interleukin-17; 25-hydroxycholesterol; inflammatory bowel disease; RORγt; γδT cells; mucosal damage;
DOI：10.19405/j.cnki.issn1000-1492.2025.07.006
〔Abstract〕 Objective To explore the role and mechanism of 25-hydroxycholesterol(25-HC) in inflammatory bowel disease(IBD) in mice. Methods All mice were divided into three groups: the control group was fed normally; the DSS model group was fed with 2.5% dextran sulfate sodium(DSS) solution; the DSS + 25-HC experimental group was fed with 2.5% DSS solution and he mice in the experimental group were intraperitoneally injected with 25-HC. The symptom changes of the mice were evaluated by assessing the disease activity index( DAI),and the tissue changes were judged by histological scoring. The expression of interleukin-17 and its signaling pathways in the mice were detected by Western blot,qRT-PCR,immunohistochemistry/fluorescence,and flow cytometry. Combined with the detection of tight junction proteins in the intestinal epithelium of the mice,the mechanism by which 25-HC affects IBD in mice was explored. Results In comparison to the DSS control group,The DSS + 25-HC experimental group mice exhibited a reduction in body weight(F = 30. 1,P