〔Abstract〕 Objective To explore the effects of the antihypertensive drug Amlodipine on calcium influx and autophagy in human podocytes(HPC). Methods HPC cells were routinely cultured in vitro. HPC cells were treated with angiotensin Ⅱ(Ang Ⅱ), the L-type Ca2+ blocker Amlodipine alone or in combination. The Ca2+ imaging system was used to detect the transient changes in the intracellular Ca2+ flux of HPC cells in real time after drug treatment. Western blot was employed to detect the changes in the ratio of autophagy marker proteins LC3B-Ⅱ/LC3B-Ⅰ, and the expression levels of Beclin-1, P62, as well as apoptosis-related proteins Bcl-2 and Bax. Flow cytometry was used to detect the number of Fluo-4AM positive cells at 488 nm to analyze the level of intracellular Ca2+ influx in HPC cells. Lyso-Tracker Green live cell staining was applied to analyze the fluorescence intensity of lysosomes. Flow cytometry was also used to detect the apoptosis rate of HPC cells. Results Compared with the control group, in the Ang Ⅱ group, the transient Ca2+ flux and the number of Fluo-4AM positive cells increased significantly(P