Found programs: National Natural Science Foundation of China (No. 82060540);Graduate Education Innovation Plan Project of Guangxi(No. JGY2024318)
Authors：Ma Beibei; Cheng Lianyu; Zhang Zhongwei; Ye Guangbin; Li Yanli Ling Bo
Keywords：solasonine; lung adenocarcinoma; H1299; apoptosis;migration; STAT3;
DOI：10.19405/j.cnki.issn1000-1492.2025.07.004
〔Abstract〕 Objective To investigate the effect of solasonine regulation of the STAT3 signaling pathway on the biological behavior of lung adenocarcinoma cells. Methods H1299 cells were treated with 0.125, 0.25, 0.5 and 0.75 mmol/L solasonine, respectively. The proliferative activity of H1299 cells was detected by CCK-8. The migration and invasion ability of H1299 cells were detected by scratch, Transwell migration and invasion assay. The apoptosis level of H1299 cells was detected by flow cytometry and Hoechest 33258/PI double staining. The protein expression levels of STAT3, p-STAT3, Bcl-2, Bax, Caspase-3, Cl-Caspase-3, Snail, Slug, N-cadherin and E-cadherin in H1299 cells were detected by Western blot assay. Results Solasonine at different concentrations significantly reduced the proliferation of H1299 cells(P