〔Abstract〕 Objective To explore the biocompatibility of hydroxyapatite and dexamethasone composite coatings on the surface of TiO2nanotubes. Methods A composite coating was constructed by loading hydroxyapatite and dexamethasone on the surface of titanium nanotubes. Field emission scanning electron microscopy, atomic force microscopy, and contact angle measurement instrument was used to observe the characteristics of sample substrates, andin vitrobiological evaluation was conducted to evaluate the effect of the coating on the adhesion and proliferation of human skull osteoblasts. Results The microstructure, three-dimensional morphology and hydrophilic properties of the modified composite coating had changed. After cytological examination, it could significantly promote the adhesion and proliferation of osteoblasts. Conclusion By simulating the immersion of body fluids and the method of adding dropwise loads, the titanium nanotube-hydroxyapatite-dexamethasone composite coating is successfully constructed, which has good biocompatibility.