〔Abstract〕 Respiratory diseases represent prevalent conditions that pose a significant threat to public health . The pathogenesis of these diseases is complex involving a variety of cells and cytokines . Cell culture technology serves as a fundamental tool for investigating pathological mechanisms and drug efficacy . However , traditional monolayer cell culture fail to mimic the intricate cell-cell interactions , limiting its applicability . In contrast , cell co-culture of- fers a more physiologically relevant approach by closely mimicking the multicellular microenvironment. This ad- vancement provides a robust model for elucidating pathological pathways and evaluating pharmacological interven- tions in respiratory diseases . In this review , the applications of cell co-culture technologies in common respiratory diseases , including chronic obstructive pulmonary disease , idiopathic pulmonary fibrosis , lung cancer , asthma , and pneumonia are summarized , in order to propose strategies for the prevention and treatment of these diseases .