〔Abstract〕 Objective To investigate the effects of paeoniflorin(Pae) on acute kidney injury(AKI) and mouse renal tubular epithelial cell(mRTEC) damage induced by lansoprazole(LPZ) and cisplatin(CIS) through in vivo and in vitro experiments.Methods The C57BL/6J mice or mRTECs were divided into four groups:normal control(NC) group,NC+LPZ group,CIS group,and CIS+LPZ group.Serum creatinine(CRE) and blood urea nitrogen(BUN) levels in mice were measured,and kidney pathology was observed with HE staining.Western blot,immunohistochemistry,and immunofluorescence were used to detect the expression levels of kidney injury molecule-1(KIM-1) and receptor-interacting protein kinase(RIPK) 1,RIPK3,and mixed lineage kinase domain-like protein(MLKL).Subsequently,C57BL/6J mice or mRTECs were divided into six groups:NC group,NC+Pae group,CIS+LPZ(M) group,and CIS+LPZ+Pae(M+Pae) group.Serum CRE and BUN levels in each group were measured,kidney pathology was observed with HE staining,and ultrastructural changes in the kidney were observed with transmission electron microscopy.The KIM-1 and necroptosis-related protein expression levels were detected by Western blot,immunohistochemistry,and immunofluorescence.Results Compared with the NC group,CRE and BUN levels were elevated in the CIS group,and these levels were further increased after LPZ intervention(all P