Found programs: National Natural Science Foundation of China (No . 81660095) ; Scientific Research Project of Shihezi University (No . ZZZC2023043)
Authors：Xing Zhuoyue , Bai Juan , Gao Xin , Huang Jiarui , Xu Lihong , Gao Yinfeng
Keywords：diarrhea-predominant irritable bowel syndrome; animal model; chemerin; intestinal mucosa inflammation; wire restraint stress;
DOI：10.19405/j.cnki.issn1000-1492.2025.11.014
〔Abstract〕 Objective To investigate the correlation of chemerin levels in the serum and intestinal mucosal with intestinal mucosal inflammation in IBS-D mice.Methods A total of 128 female C57BL/6J mice were randomly divided into IBS-D group and control group,with 64 mice in each group.Wire Restraint Stress method(WRS) was used to construct an animal model of IBS-D.Enzyme-linked immunosorbent assay was used to detect serum and colonic mucosal chemerin levels in mice.Hematoxylin-eosin(HE) staining was used to observe colonic mucosal inflammation.Geboes value was used to evaluate the severity of colonic mucosal inflammation.Results In the IBSD group,the concentration of serum chemerin increased and reached the peak in the first week of the experiment(t=6.538,P