〔Abstract〕 Objective To explore the alleviating effect of geniposide on ulcerative colitis(UC) and to investigate its potential mechanism.Methods A UC mouse model was induced using 5% dextran sulfate sodium(DSS).These mice were randomly divided into 6 groups(n=8):control group,model group,sulfasalazine group [100mg/(kg·d)],low-dose geniposide group[10 mg/(kg·d)],medium-dose geniposide group[20 mg/(kg·d)],and high-dose geniposide group[40 mg/(kg·d)].The mice were orally administered for consecutive 10 days.The colon length and mouse body mass were measured,and the colon mucosal damage index(CMDI) and disease activity index(DAI) were scored.The pathological changes in colon tissue were observed using hematoxylin-eosin(HE) staining.The reagent kits were used to measure the levels of malondialdehyde(MDA),myeloperoxidase(MPO),catalase(CAT),and glutathione(GSH) in colon tissue.The enzyme linked immunosorbent assay(ELISA) was used to analyze the expression levels of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),and IL-1 β in colon tissue.Western blot was used to detect the protein expression of mucin 1(MUC-1),occludin,IL-6,p-JAK2,and p-STAT3 in colon tissue.Results Compared with the normal control group,the body mass and colon length of the model group mice significantly reduced.The expression of MUC-1 and occludin proteins significantly reduced(P