〔Abstract〕 Objective To analyze differences in the expression levels of lipid metabolism-related proteins in adipose tissue exosomes between obese mice and wild-type mice using proteomic techniques.Methods Wild-type(WT) and obese(ob/ob) model mice of the same age were selected,with 8 mice per group.Adipose tissue from both groups was minced and cultured for 48 hours.Conditioned media was collected,and exosomes were isolated using differential centrifugation.Liquid chromatography-tandem mass spectrometry(LC-MS/MS) was utilized for proteomic analysis to screen differentially expressed proteins(DEPs).Gene ontology(GO) enrichment analysis and kyoto encyclopedia of genes and genomes(KEGG) pathway enrichment analysis were performed on the DEPs.Heatmaps were generated to visualize DEPs expression patterns,and Western blot was employed to validate DEPs expression levels.Results Exosomes were successfully extracted from the culture supernatant of adipose tissues from mice in the WT group and the ob/ob group.Mass spectrometry analysis identified a total of 25 629 peptides and 3 376 proteins.Compared with the WT group,there were 699 proteins with high expression and 632 proteins with low expression in the exosomes derived from adipose tissues of ob/ob mice.Both GO and KEGG analyses showed that DEPs were mainly enriched in metabolic pathways.Heatmap analysis visualized the expression patterns of metabolism-related DEPs,and the expression levels of lipid metabolism-related proteins such as acyl-Co A synthetase long-chain family member 1(ACSL1),apolipoprotein E(ApoE),and albumin(Alb) changed significantly in the obese state(P