Found programs: Scientific Research Project of Hebei Provincial Administration of Traditional Chinese Medicine (No . 2021071)
Authors：Li Min , Yang Menghua , Gao Yi , Zhang Zijian , Jiang Dan
Keywords：Anemoside B4; nitric oxide synthase 3; dihydrofolate reductase; glutamine metabolism; oral lichen planus;
DOI：10.19405/j.cnki.issn1000-1492.2025.11.010
〔Abstract〕 Objective To investigate the mechanism of Anemoside B4(AB4) on glutamine metabolism in oral lichen planus(OLP) epithelial cells via the nitric oxide synthase 3(NOS3)-dihydrofolate reductase(DHFR) axis.Methods Bioinformatics analysis was performed to identify the intersection of molecular targets of OLP,AB4,and genes related to glutamine metabolism.A lipopolysaccharide(LPS)-induced HOK-16B model of OLP was established.HOK-16B were divided into Ctrl group,OLP group,AB4 group,OLP+oe-NOS3 group,OLP+sh-NOS3group,OLP+sh-NOS3+oe-DHFR group,and OLP+sh-NOS3+AB4 group.Cell proliferation was detected by cell counting kit-8(CCK-8);cell apoptosis was detected by TdT-mediated dUTP Nick-End Labeling(TUNEL);inflammatory factors ilnterleukin(IL)-1β,tumor necrosis factors-α(TNF-α) concentrations in cell supernatants were measured using enzyme-linked immunosorbent assay(ELISA) kits;glutamine uptake and glutamate production were determined using kits;and the protein expression of alanine-serine-cysteine transporter2(ASCT2) and glutamine synthase(GLS) was assessed by Western blot.Results Bioinformatics analysis of molecular targets of OLP,AB4,and genes related to glutamine metabolism revealed three intersection targets:NFE2L2,NOS1,and NOS3.Compared with the Ctrl group,the OLP group exhibited decreased HOK-16B cell viability(P