〔Abstract〕 Objective To investigate the role of Ras-GTPase-activating protein SH3 domain-binding protein 2 （G3BP2）in regulating the activation，proliferation，and migration of hepatic stellate cells（HSCs）. Methods The mouse HSCs（JS-1 cell line）were treated with 5 μg/L transforming growth factor-beta 1（TGF-β1）for 24 hours to establish an HSC activation and proliferation model. A G3BP2 knockdown system was constructed using siRNA in ⁃ terference technology. The experiment was divided into four groups：Control，TGF-β1 treatment，TGF-β1+si-NC， and TGF-β1+ G3BP2-siRNA. The expression levels of key fibrosis indicators，including type I collagen（Collagen I），α-smooth muscle actin (α-SMA），and G3BP2，were detected by Western blot and RT-qPCR. Cell proliferation activity was assessed using the CCK-8 proliferation assay kit and EdU fluorescence labeling technology. Cell migra⁃ tion ability was analyzed by scratch wound healing assay and Transwell migration assay. The formation level of stress granules was quantified by immunofluorescence microscopy to investigate the effects of G 3BP2 on stress gran⁃ ule formation in activated HSCs. Results Stimulation with TGF-β1 upregulated the expression of G3BP2 in JS-1 cells（RT-qPCR：P<0. 000 1；Western blot：P<0. 000 1），while a downward trend in its expression was observed in the G3BP2⁃silenced group（RT-qPCR：P<0. 01；Western blot：P<0. 000 1）. Compared with the control group， the TGF- β1 group exhibited increased protein expression levels of α -SMA and Collagen I（RT-qPCR：both P< 0. 01；Western blot：P<0. 01 and P<0. 05，respectively），concomitant with an increased number of stress granules and enhanced cell proliferation and migration capacity（all P<0. 001）. The experimental results demonstrated that G3BP2 knockout effectively reversed the aforementioned phenotypes，with the G3BP2-silenced group showing re⁃ duced expression of fibrotic markers（all P<0. 01）， decreased stress granule formation（P<0. 01）， and reduced cell proliferation and migration capacity（all P< 0. 05）， compared to the negative control group. Conclusion G3BP2 enhances the activation，proliferation，and migration of HSCs by promoting the formation of stress gran ⁃ ules，thereby accelerating the pathological progression of liver fibrosis. This suggests that stress granules may serve as important regulators in controlling the activation，proliferation，and migration of HSCs.