〔Abstract〕 Objective To study the effects of sorafenib on the proliferation, cycle and apoptosis of human intestinal cancer RKO cells. Methods RKO cells were treated with sorafenib at different concentrations(0.5,1.0,5.0,10.0,20.0,40.0 μmol/L),and the inhibitory effect of sorafenib on the proliferation of RKO cells was detected by MTT method, and the half inhibition of concentration(IC50) was determined, set as the experimental group.The control group was treated with dimethyl sulfoxide.For the two groups of cells, the cell clone formation plate method was used to detect the proliferation ability, the flow cytometry was used to detect cell apoptosis and cell cycle changes, and the expression level of intracellular apoptosis-related proteins Caspase-3,Caspase-8 and PARP were detected by Western blot. Results Sorafenib had an inhibitory effect on the proliferation of RKO cells in a concentration-dependent manner.The IC50of sorafenib on RKO cell lines was 10 μmol/L.Compared with the control group, sorafenib(10 μmol/L) could inhibit the clone formation ability of RKO cells(P<0.001) and increase the apoptosis rate[(32.53±6.64)%vs(4.47±2.09)%,P<0.01],affect the cell cycle(P<0.01) and promote the expression levels of apoptosis-related proteins Caspase-3,Caspase-8 and PARP(P<0.05). Conclusion Sorafenib has the ability to inhibit the proliferation of human colorectal cancer RKO cells, and its mechanism may be related to promoting RKO cells apoptosis and affecting the cell cycle.