〔Abstract〕 Objective To investigate the protective effect of adiponectin APN on myocardial injury caused by sepsis and its possible mechanism. Methods According to the experimental design, 30 healthy C57 BL/6 male mice were randomly divided into 3 groups: sham operation group(sham group), lipopolysaccharide treatment group(LPS group), adiponectin treatment group(APN+LPS group). HE staining was used to observe the injury of mouse heart. Immunohistochemical staining and Western blot were used to observe the expression of connexin 43(Cx43), Caspase3, Bax, Bcl-2 proteins. Results Compared with sham group, the expression of Bax, cleaved Caspase3 protein increased and the expression of Bcl-2 protein decreased in LPS group. Compared with LPS group, the injury in APN+LPS group was significantly alleviated, showing that the expression of Bax, cleaved caspase3 protein decreased and the expression of Bcl-2 protein increased. Meanwhile, compared with sham group, the expression of Cx43 increased in LPS group and decreased in APN+LPS group. Conclusion Adiponectin can attenuate LPS-induced cardiac injury in septic mice. The mechanism may be through the inhibition of apoptotic signal pathway and the expression of Cx43.