〔Abstract〕 Objective To investigate the expression of long chain non-coding RNA FAS-AS1 in glioma cells and its effect on the proliferation, migration and invasion of glioma cells. Methods The abundance of FAS-AS1 expression in clinical samples and glioma cell lines was detected by qRT-PCR. Human glioma cell lines U251 and SF126 were transfected with FAS-AS1 plasmid. The transfection efficiency was detected by qRT-PCR, the effect of FAS-AS1 on cell proliferation was detected by MTT formation test and cloning test, the effect of FAS-AS1 on cell migration was detected by Transwell assay, and the effect of FAS-AS1 on glioma cell invasion was detected by matrix gel biofilm combined with Transwell experiment. Results The expression of FAS-AS1 in glioma tissue and human glioma cell line was down-regulated; the glioma cell model with overexpression of FAS-AS1 was successfully constructed by FAS-AS1 plasmid transfection, and the overexpression of FAS-AS1 inhibited the proliferation, migration and invasion of glioma cells. Conclusion FAS-AS1 is down-regulated in gliomas and can inhibit the proliferation, migration and invasion of glioma cells.