〔Abstract〕 To investigate the relationship between proteasome 20 S subunit beta 8(PSMB8) mRNA expression and clinical characteristics and prognosis of glioma patients. 325 patients with gliomas were from CGGA. PSMB8 mRNA was enriched in high-grade glioma and isocitrate dehydrogenase 1 wild-type(IDH1-wt) group. Pearson correlation test showed that PSMB8 was correlated with other PSMB8 genes. PSMB8 mRNA expression was positively correlated with malignant phenotype and negatively correlated with overall survival(OS), suppression of PSMB8 may be taken as a new strategy for the treatment of PSMB8 over expressing gliomas.