〔Abstract〕 Objective To analyze the breeding and genotype of Agtr2-/-mice by constructing Angiotensin Ⅱ type 2 receptor(Agtr2-/-) mice with CRISPR/Cas9 technique. Methods Agtr2-/-F0 generation mice were obtained by using CRISPR/Cas9 gene targeted knockout technology. The genotypes of mouse tail were identified by PCR. Agtr2-/-mice of F1 generation were procured from F0 generation Agtr2-/-mice mating with the same litter wild-type mice after sexual maturity. Western blot was used to check on the dependability of PCR identification results from protein levels. Results The Agtr2-/-mice were successfully constructed by CRISPR/Cas9 technology. The obtained mice were bred and genotypes were identified. Agtr2+/+, Agtr2+/-, and Agtr2-/-stable Agtr2 gene mice were obtained. Western blot results confirmed that Agtr2 protein was almost not expressed in the heart, spleen, thymus, liver and kidney of Agtr2-/-mice. Conclusion The Agtr2-/-mouse model was successfully constructed through CRISPR/Cas9 technology, and the homozygous Agtr2-/-mice were obtained through reliable identification methods and appropriate breeding methods.