〔Abstract〕 Objective To investigate the effects of high-salt environment on store-operated calcium entry(SOCE) and contractile function in rat coronary artery smooth muscle. Methods After culturing the isolated rat coronary arteries in different concentrations of high-salt culture media(with 137.65～167.65 mmol/L NaCl respectively) for 24 h, the distribution and expression of Orai1, STIM1, IP3R and ETAin coronary artery smooth muscle were detected by immunohistochemistry assays, U46619, ET-1 and SOCE-induced coronary vasoconstriction were examined by microvascular tension measurement system, and the SOCE mediated Ca2+influx in RCASMCs was detected by calcium imaging technology. Results High-salt environment significantly enhanced ET-1 and SOCE-induced coronary vasoconstriction(P<0.01) and SOCE mediated Ca2+influx in the RCASMCs(P<0.01), and increased the expression of Orai1, STIM1 and IP3R in coronary artery smooth muscle(P<0.05). Conclusion The extracellular high-salt environment can enhance the contractile function of rat coronary artery induced by agonist and SOCE, and the mechanism may be associated with the up regulation of key proteins related to the IP3R-SOCE pathway and the enhancement of Ca2+influx mediated by SOCE in the rat coronary artery smooth muscle.