〔Abstract〕 Objective To investigate the expression of miR-513 a-3 p in colorectal cancer tissues and adjacent normal tissues, and to explore the mechanism of miR-513 a-3 p inducing colorectal cancer(CRC) cell growth and migration. Methods The total of 30 patients with colon cancer were selected as the research objects. The expression levels of miR-513 a-3 p in colon cancer tissues and adjacent tissues were detected by in situ hybridization and real-time fluorescence quantitative PCR. CRC cell lines HT-29 and SW480 were cultured and transfected with miR-513 a-3 p analogues as the expression promoting group. The negative control group was set up. The cell viability was detected by tetramethyl azo salt(MTT) colorimetry, the cell invasion was detected by Transwell method and cell migration was detected by cell scratch test. The expression of NF-κB and Wnt/β-Catenin pathway related proteins was detected by Western blot. Results The results of situ hybridization showed that the positive expression of miR-513 a-3 p in colon cancer tissues was higher than that in adjacent tissues. The results of qRT-PCR showed that the relative expression of mir-513 a-3 p was(0.37±0.09) in colon cancer tissues and(1.01±0.16) in adjacent tissues, and the difference was statistically significant(P<0.01). Compared with the negative control group, the cell viability of the promoted expression group increased in 3 and 4 d(P<0.01), the invasion rate of HT-29 and SW480 cells in the promoting expression group increased(P<0.01), and the cell migration ability of HT-29 and SW480 in the promoting expression group was enhanced. The results of Western blot showed that compared with the negative control group, the relative expressions of p-p65, p-IκBα, Wnt3 a, Wnt5 a and β-Catenin protein in HT-29 cells and SW480 cells significantly increased(P<0.01). Conclusion The overexpression of miR-513 a-3 p promotes the growth, migration and invasion of colon cancer cells. The possible mechanism is related to the activation of NF-κB pathway and Wnt/β-Catenin pathway.