〔Abstract〕 Objective To investigate the characteristics of gait disorders in patients with cerebral small vessel disease(CSVD) and the correlation between the number and location of cerebral microbleeds and gait disorders. Methods 102 patients with cerebral small vessel disease were enrolled and divided into CSVD with cerebral microbleeds group(n=29) and CSVD without cerebral microbleeds group(n=73). In addition, age and sex-matched patients who without CSVD on cranial MRI were selected as the control group(n=30). The quantitative gait parameters of all subjects were measured, and the differences of gait parameters between cerebrovascular disease group and normal control group were compared. The gait parameters included step length, gait speed, stride frequency, and the percentage of bipedal support phase. The correlation between the number and location of cerebral microbleeds and gait parameters such as step length, gait speed, gait frequency and the percentage of bipedal support phase were statistically analyzed. Results Compared with the normal group, CSVD with cerebral microbleeds group was significantly shorter and slower(P<0.05) while CSVD without cerebral microbleeds group was shorter and faster(P<0.05). There was no significant difference between CSVD with cerebral microbleeds and CSVD without cerebral microbleeds. The number of cerebral microbleeds was negatively correlated with step length(r=-0.295,P=0.001) and positively correlated with foot support(r=0.225,P=0.011), the more the number of cerebral microbleeds, the shorter the step length and the longer the support phase of feet. The cerebral microbleeds in frontal lobe, temporal lobe and deep part were associated with shorter step length and slower walking speed(P<0.05). Conclusion As an independent factor, cerebral microbleeds are associated with gait disorders in patients with cerebrovascular diseases. Frontal lobe, temporal lobe and deep cerebral microbleeds are correlated with gait disorders.