〔Abstract〕 Objective To analyze the expression of Lnczc3 h7 a in colorectal cancer cells and its effect on the proliferation and migration of colorectal cancer cells, and explore its potential mechanism. Methods Six colorectal cancer cell lines including SW620、SW480、HCT116、DLD-1、Caco-2、HT-29 and normal colorectal epithelial cell FHC were selected. The expression of Lnczc3 h7 a in colorectal cancer cell lines and normal colorectal epithelial cells was detected by RT-PCR. Lnczc3 h7 a mimics and Lnczc3 h7 a inhibitor were used to transfect colorectal cancer cells SW620 respectively and named as Lnczc3 h7 a mimics group and Lnczc3 h7 a inhibitor group respectively, and the untransfected cells were used as the control group.CCK-8 method and cell scratch test were used to detect the difference of cell proliferation and migration ability among the three groups, and Western blot method was used to detect the effect of Lnczc3 h7 a on the expression of CTHRC6 protein in colorectal cancer cells. Results The results of RT-PCR showed that the relative expression levels of Lnczc3 h7 a in the six colon cancer cell lines were significantly lower than those of normal colorectal epithelial cells FHC, and all were statistically significant(P<0.05). CCK-8 test results showed that the cell proliferation ability of the Lnczc3 h7 a mimics group was significantly lower than that of the Lnczc3 h7 a inhibitor group and the control group(P<0.05), and the cell proliferation ability of the Lnczc3 h7 a inhibitor group was significantly higher than that of the control group after 4 and 5 days of transfection. The scratch test results showed that the difference of wound healing rate among the three groups was statistically significant(F= 395.524, 480.165,P<0.05); compared with the control group, the cell migration ability of the Lnczc3 h7 a mimics group significantly reduced(P<0.05), and the cell migration ability of the Lnczc3 h7 a inhibitor group significantly increased(P<0.05). Western blot results showed that compared with the control group, the expression of CTHRC6 protein in the Lnczc3 h7 a inhibitor group significantly increased(P<0.05), and the expression of CTHRC6 protein in the Lnczc3 h7 a mimics group significantly reduced(P<0.05). Conclusion Lnczc3 h7 a may inhibit the proliferation and migration of colorectal cancer cells by targeting CTHRC6.