〔Abstract〕 Objective To study the effects of GDF-associated serum protein-1(GASP-1) gene interference on malignant invasion, epithelial-mesenchymal transition(EMT) and microtubule formation in non-small cell lung carcinoma(NSCLC) cells. Methods Lung cancer A549 cells were used as a model. The shRNA1 screened in the previous experiment was transfected into lung cancer A549 cells with Lipofectamine 2000. Grouping: control group, shRNA group and GASP-1-shRNA1 group. Transwell test was used to detect cell invasion ability, scratch test was used to detect cell migration ability; microscopic observation was used to observe EMT; microtubule formation test was used to detect the number of microtubule nodules; Western blot was used to detect EMT markers E-cadherin, N-Expression of cadherin, Fibronectin and VEGF protein. Results Compared with the control group, the number of cell invasions per field in the GASP-1-shRNA1 group significantly reduced, and the rate of scratch healing significantly reduced, indicating that interference with the GASP-1 gene could significantly inhibit the invasion and migration of A549 cells. Compared with the control group, the EMT in the GASP-1-shRNA1 group was significantly inhibited. In the microtubule formation experiment, compared with the control group, the number of microtubule nodules in the GASP-1-shRNA1 group significantly reduced, and the expression level of vascular endothelial growth factor(VEGF) protein significantly reduced. Conclusion GASP-1 gene interference can significantly inhibit the malignant invasion, EMT and microtubule formation of NSCLC cells.