〔Abstract〕 Objective To investigate the effects of placenta derived exosomes on the proliferation, invasion, migration and tube formation capacity of vascular endothelial cells in gestational diabetes mellitus(GDM). Methods Placental exosomes were obtained by using the method ofin vitroculture of placental mass and hypervelocity centrifugation. Transmission electron microscopy(TEM), dynamic light scattering(DLS) and Western blot were used to identify those exosomes; Human umbilical vein endothelial cells(HUVEC) were culturedin vitro. The uptake of exosomes by HUVEC was detected by fluorescence confocal microscopy after double staining; CCK-8 and EdU, scratch and Transwell invasion and tube formation assay were used to detect the effects of different concentrations of exosomes on the proliferation, migration, invasion and angiogenesis of endothelial cells. Results The placental exosomes with diameters ranging from 40 to 130 nm were successfully isolated. The isolated and purified placenta exosomes could be absorbed by endothelial cells and could promote the proliferation, migration, invasion and tube forming ability of endothelial cells in a concentration dependent manner. Conclusion The exosomes from placental tissue of gestational diabetes mellitus can promote the proliferation, migration, invasion and tube forming ability of endothelial cells.