〔Abstract〕 Objective To explore the effects of interfering with long non-coding RNA CCAT2(lncRNA CCAT2) on mitochondrial function of bladder cancer cells J82 and features of tumor stem cells. Methods The expression of CCAT2 was interfered by constructing lncRNA CCAT2 short hairpin RNA(shRNA),which was verified by RT-PCR.J82 cell lines with the lowest expression level of CCAT2 were selected for the subsequent experiments.The cell proliferation was detected by clone formation assay.The changes in mitochondrial membrane potential(MMP) were detected by flow cytometry.The levels of apoptosis-related proteins were detected by Western blot.The pelletization of tumor cells was observed under microscope.The expression of CD133 in each group was detected by flow cytometry.The levels of OCT4,SOX2 and CD44 were detected by Western blot. Results The expression level of CCAT2 in three kinds of CCAT2-shRNA J82 cell lines was lower than that in shRNA-NC(P<0.05).The colony formation rate,level of c-Myc,tumor pelletization diameter and pelletization rate,number of positive CD133 cells,and levels of OCT4,SOX2 and CD44 in CCAT2-shRNA group were lower than those in shRNA-NC group(P<0.05),while green fluorescence intensity,Bax/Bcl-2,levels of Caspase-3 and Caspase-9 were higher than those in shRNA-NC group(P<0.05). Conclusion Down-regulating the expression level of lncRNA CCAT2 can inhibit the growth of J82 cells,which may be related to the influence of cellular mitochondrial function and tumor stem cell-like features.