〔Abstract〕 Objective To study whetherrotavirus(RV) can promote the phosphorylation of signal transduction activator 3(STAT3),induce intestinal epithelial cells to secrete antimicrobial peptide RegⅢ-γ, and regulate intestinal inflammation after infection of BALB/c suckling mice. Methods 7-day-old SPF grade BALB/c suckling mice were administrated with RV by gavage.The experiment was divided into five groups:normal control group,PBS control group,RV infection group,RV + STAT3 inhibitor group and STAT3 inhibitor group.Five suckling mice were killed at 1,2,3,4,5,6,7,8,9 and 10 days after infection,respectively.Enzyme linked immunosorbent assay(ELISA) was used to detect the change of IL-22 content in intestinal homogenate,Western blot was used to detect the expression of STAT3,pSTAT3 and RegⅢ-γ protein at different time points in each group. Results After RV infection,the intestinal tissue showed obvious inflammatory reaction,and the level of IL-22 increased gradually with the infection time which reached the maximum value on the fourth day of infection.The protein levels of pSTAT3 and RegⅢ-γ showed the similar change trend with IL-22,while the protein level of STAT3 showed the opposite trend,and the expression level was the lowest on the fourth day after infection. Conclusion RV can activate IL-22/pSTAT3/RegⅢ-γ signaling pathway and regulate the development of intestinal inflammation.