〔Abstract〕 Objective To construct a human derived pEGFP-C1-p38γ expression plasmid and observe its expression of inflammatory factors in alcohol-induced L0-2 cells and its effect on cell proliferation and apoptosis. Methods RNA was extracted from L0-2 cells and reversely transcribed into cDNA. At the same time, the primers were diluted to 10 μmol/L and the rest were used as storage fluid. P38γ was amplified by PCR technology and identified. The DNA gel recovery kit AxyPrep was used for the purification and recovery of PCR products. Then the PCRproducts and carriers were recovered by enzyme digestion. After enzyme digestion and identification, they were sent to sequencing for identification and sequencing. The constructed plasmids were transfected into alcohol-induced L0-2 cells of human mononuclear macrophage cell line. The effects on cell proliferation and apoptosis were detected by MTT assay and flow cytometry, and the expressions of inflammatory cytokines IL-6 and TNF-α in alcohol-induced L0-2 cells were detected by Western blot. Results Sequencing results showed that pEGFP-C1-p38γ eukaryotic expression plasmid was successfully constructed. The results of MTT assay showed that 24 h later, the cell proliferation rate of the p38γ overexpressed group(0.42±0.08) % was significantly lower than that of the control group(0.60±0.03)%. The results of flow cytometry showed that the apoptosis rate of p38γ overexpressed cells group was(17.46±1.52) %, significantly higher than that in the control group(13.18±1.34) %(P<0.05). Western blot result showed that the expression of IL-6 and TNF-α in L0-2 cells transfected with pEGFP-C1-p38γ plasmid was higher than that in the control group. Conclusion P38γ can significantly inhibit the proliferation and promote the apoptosis of alcohol-induced L0-2 liver cells. P38γ promotes the expression of inflammatory cytokines IL-6 and TNF-α in alcohol-induced L0-2 cells.