〔Abstract〕 Objective To explore the effect of Arctigenin on renal ischemia-reperfusion injury model in aged rats. Methods The model of renal ischemia-reperfusion injury in elderly rats was established, and the rats were randomly divided into 6 groups by random number table method: Sham group, Arctigenin group(ACR), renal ischemia-reperfusion model group(RIRI), RIRI+ACR(10 mg/kg) group, RIRI+ACR(20 mg/kg) group and RIRI+ACR(50 mg/kg) group for follow-up experiments. Kit was used to detect Scr and BUN levels. HE staining was used to detect pathological damage of renal tissue. Apoptosis was detected by TUNEL staining. The levels of inflammatory cytokines TNF-α, IL-6 and IL-8 were detected by ELISA. The expression levels of KIM-1, NGAL, cleaved caspase-3, cleaved caspase-9, PI3 K, p-PI3 K, Akt and p-Akt were detected by Western blot. After the PI3 K/Akt pathway inhibitor LY294002 was added, the rats were randomly divided into 5 groups: Sham group, RIRI group, RIRI+ACR(50 mg/kg) group, RIRI+LY294002 group and RIRI+ACR+LY294002 group. Western blot was used to detect the expression levels of PI3 K, p-pi3 k, Akt and p-akt in the rats in each group. The kit was used to detect the levels of Scr and BUN, and ELISA was used to detect the levels of TNF-α, IL-6 and IL-8. Results The results showed that compared with RIRI group, RIRI+ACR(20, 50 mg/kg) group of Scr and BUN levels in rats decreased obviously, obviously improved pathological damage degree, KIM-1, NGAL protein levels significantly decreased, apoptotic cells significantly reduced, cleaved caspase-3, cleaved caspase-9 protein levels significantly decreased, the TNF alpha, IL-6, IL-8 levels significantly decreased, p-PI3 K/PI3 K, p-Akt/Akt ratio increased significantly. Conclusion Arctigenin has protective effect on renal ischemia-reperfusion injury in aged rats.