〔Abstract〕 Objective To investigate the effect of sevoflurane on cerebral ischemia-reperfusion injury(CIRI) and the role of silent information regulator 1(SIRT1) as well as autophagy. Methods SD rats were divided into sham group, middle cerebral artery ischemia(MCAO) model group, sevoflurane(0.6%, 1.2%, 2.4%) treatment groups, rats were pretreated with different dose of sevoflurane, middle cerebral artery occlusion was used to establish model and neurological score was evaluated, the infarct volume was measured by TTC staining, and the brain water content was assessed, HE staining was used to detect the pathological change, cell apoptosis rate was measured by TUNEL assay, the contents of tumor necrosis factor-α(TNF-α), interleukin-6(IL-6), interleukin-10(IL-10) were measured by ELISA assay, SIRT1 gene expression was detected by RT-PCR, the protein levels of SIRT1,LC3 and p62 were detected by Western blot. Autophagy inhibitor 3-MA and SIRT1 siRNA were used to treat rats,and their effects on CIRI injury were examined. Results Compared with sham group,the MCAO model rats appeared neurological deficit,infarct volume rate increased,brain water content increased,significant pathological changes and apoptosis in the cerebral cortex appeared,inflammation increased,SIRT1 gene and protein expression increased,level of autophagy increased; sevoflurane pretreatment could alleviate the cerebral ischemiareperfusion injury,meanwhile,the SIRT1 expression and autophagy levels were further elevated. The autophagy inhibition of 3-MA and SIRT1 silence could reverse the protective effect of sevoflurane in cerebral ischemia-reperfusion,and decrease the level of autophagy. Conclusion Sevoflurane can attenuate cerebral ischemia-reperfusion injury in rats by inducing SIRT1-dependent autophagy.