〔Abstract〕 Objective To explore the specific mechanism of LncRNA NRON affecting NFAT activity and progression of atrial fibrosis. Methods The expression level of NRON in atrial tissue was detected by qRT-PCR. Western blot was used to determine the protein levels of collagen Ⅰ, collagen Ⅲ, NFATc3 and pNFATc3. Immunohistochemistry was performed to observe the expression and distribution of collagen Ⅰ in atrial tissue. Atrial fibroblasts were identified by vimentin/troponin immunofluorescence staining. Then the proliferation of fibroblasts was detected by CCK-8 analysis. HE staining was used to observe the morphological changes of cardiac tissue, and finally the myocardial fibrosis was detected by Masson staining. Results NRON inhibited fibroblast proliferation, and the expression of collagen Ⅰ and collagen Ⅲ, activated NFATc3 and nuclide entry. Overexpression of NRON inhibited the cardiac fibrosis induced by NFAT in mice. Conclution The data from this study suggest that NRON alleviates atrial fibrosis by promoting NFATc3 phosphorylation.