〔Abstract〕 Objective To prepare Kaposiʼs sarcoma-associated herpesvirus(KSHV) cell antigen tablets and verify their effectiveness, providing a feasible method for the detection of KSHV serum samples. Methods BCBL-1 cells stably expressing KSHV nuclear-associated antigen(LANA) were cultured. BCBL-1 cells were cells infected with KSHV and contained the KSHV gene in their genome. LANA is a protein that is highly expressed on the nucleus of a host cell. When the cells were vigorously viable and in good condition, the cells were collected and fixed to the pre-treated antigen tablets with 4% paraformaldehyde. After blocking,incubation of the serum to be tested and incubation of the fluorescent secondary antibody,the results were observed by immunofluorescence microscopy. Results The fluorescence of the fluorescent secondary antibody was observed in the serum samples of patients infected with KSHV by immunofluorescence technique,while the fluorescence of the secondary antibody was not observed by the specimens not infected with KSHV. Conclusion The cell antigenic tablets of KSHV which can detect serum have been successfully prepared,which provides a method for further studying the epidemiology of KSHV.