〔Abstract〕 Objective To compare the related properties of human dental pulp stem cells(DPSCs) and pericytes,and to investigate whether DPSCs can be used to replace pericytes for vascular regeneration. Methods DPSCs and pericytes were induced into osteogenesis and neurogenic differentiationin vitroto identify the multidirectional differentiation potential. Matrigel three-dimensional culture technique was used to evaluate the ability of DPSC and pericytes to stabilize vascular-like structure. Transwell assay was performed to evaluate the induced capacities of DPSCs and pericytes on the migration behavior of human umbilical vein endothelial cell(HUVEC). Results DPSCs and pericytes showed similar morphology and could differentiate into osteogenic and neurogenic cells after induction. The two kinds of cells co-cultured with HUVEC on the Matrigel could establish stable vascular-like structure. In Transwell assay, DPSC and pericytes showed strong induced capacities on the migration behavior of HUVEC. Conclusion DPSCs have similar morphological and functional characteristics to pericytes and can be used for vascular regeneration.