〔Abstract〕 Objective To investigate the effect of CD147 on sensitivity of prostate cancer cells to docetaxel(DOC). Methods The experiment included negative control group(PC-3/Scramble) and CD147 shRNA group(PC-3/shCD147). PC-3/Scramble and PC-3/shCD147 cells were treated with DOC for 72 h. The cell proliferation was detected by MTT assay. The cell apoptosis was determined by flow cytometry. The protein expressions of β-catenin and P-glycolprotein(P-gp) were detected by Western blot. Results IC50values of DOC treatment in PC-3/Scramble cells(12.47±1.71) μmol/L were higher than those in PC-3/shCD147 cells(2.38±0.13) μmol/L. The 5 μmol/L DOC induced apoptosis in PC-3/Scramble cells(8.75±0.75)% was lower than that in PC-3/shCD147 cells(16.1±0.95)%(P<0.01). The expression of β-catenin and P-gp protein in PC-3/shCD147 cells was significantly lower than that in PC-3/Scramble cell(P<0.01). Wnt pathway agonist LiCl-treated PC-3/shCD147 cells showed the increase in cell survival(P<0.01) and expression levels of β-catenin and P-gp(P<0.01) compared with PC-3/shCD147 cells. Conclusion CD147 decreases the sensitivity of prostate cancer cells to DOC via Wnt/β-catenin pathway.