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Objective To study the regulation of CP-25 on P-glycoprotein(P-gp) expression and function in Caco-2 cells. Methods Human colon cancer cell line Caco-2 was culturedin vitro. The apparent permeability coefficient(Papp) was used as an indicator to study the effect of pH on the transport of CP-25. Flow cytometry was used to detect the expression and activity of CP-25 on P-gp. The expression of Phospho-NF-кB p65 and P-gp in Caco-2 cells was detected by Western blot. The expression of Phospho-NF-кB p65 in Caco-2 cells was detected by laser confocal microscopy. Results The Pappvalue indicated that CP-25 was higher in the colon than in Pae. The results of flow cytometry showed that CP-25 significantly increased the accumulation of RHO123 in cells and down-regulated the expression of P-gp. Western blot results showed that CP-25 could significantly down-regulate the expression of Phospho-NF-кB p65 and P-gp after treatment. Laser confocal assay showed that CP-25 could down-regulate the expression of Phospho-NF-кB p65. Conclusion CP-25 can down-regulate the expression of P-gp in Caco-2 cells and inhibit its transport activity, which may be related to the activation of NF-кB signaling pathway.

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Objective To investigate the functions of LIM-domain protein 4(LMO4)in regulating CD133 expression and proliferation of prostate cancer cells and its mechanism. Methods Immunohistochemistry(IHC)was used to detect the expression of LMO4 and CD133 in prostate cancer tissues and prostatic hyperplasia tissues. Fluorescence activated cell sorting was used to harvest CD133+and CD133-cells. Colony formation assay was performed to determine proliferation ability. RNA interference was performed to knockdown Lmo4 expression in PC-3 cells.Western blot and immunofluorescence assay were used to detect the expression of LMO4 and CD133 protein in PC-3 cells or PC-3/Si Lmo4 cells. Flow cytometry tested the CD133+cells number and cells proliferation ability. Colony formation assays were used to determine PC-3 cells clonogenic capability. Co-Immunoprecipitation( Co-IP) was used to detect the interaction between LMO4 and cyclin-dependent kinase 9( CDK9). Results Comparing to prostatic hyperplasia tissues,CD133 and LMO4 expresssions dramatically increased in prostate cancer tissues. CD133+subpopulation of PC-3 cells exhibited high clonogenic capability. Both CD133 expression and numbers of CD133+cells significantly decreased in LMO4 knockdown PC-3 cells. Furthermore,silencing Lmo4 led to decrease the abilities of cell proliferation and clonogenic. Co-immunoprecipitation analysis showed that LMO4 and CDK9 could form a LMO4/CDK9 complex in prostate cells. However,CDK9 may leave from the complex owing to LMO4 overexpression in prostate cells. Interestingly,the LMO4/CDK9 complex was not detected in PC-3/Si Lmo4 cells. Conclusion Up-expression of the nuclear transcription factor LMO4 is consistent with higher expression of cancer stem marker CD133 in prostate cancer. Prostate cancer cells proliferation may be regulated by LMO4 in an independent-LMO4/CDK9 complex manner.

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Objective To investigate the effect of insulin-like growth factor Ⅱ(IGF-Ⅱ) methylation on trophoblast cells apoptosis induced by high glucose and PI3 K/Akt pathway. Methods Human choriotrophoblast cell line HTR-8/SVneo was culturedin vitroand randomly divided into control group, high glucose group, 5-Aza-2′-deoxycytidine(methyltransferase inhibitor)+high glucose group(50 mmol/L of glucose). High glucose group and 5-aza-dc+high glucose group were cultured in high glucose medium, then 5-aza-dc+high glucose group was treated with 5-aza-dc. The methylation level of IGF-Ⅱ was detected by nested methylation specific PCR(nMS_PCR). The cell proliferation was detected by CKK-8. The cell apoptosis rate was detected by flow cytometry. The expressions of IGF-Ⅱ protein, apoptosis-related protein(Bcl-2, Bax, caspase-3), PI3 K/Akt pathway protein(PI3 K, p-PI3 K, Akt, p-Akt) were detected by Western blot. Results Compared with the control group, the cell relative survival rate and the expressions of IGF-Ⅱ, Bcl-2, p-PI3 K and p-Akt proteins in the high glucose group decreased(P<0.05); the methylation level of IGF-Ⅱ, apoptotic rate, expressions of Bax and caspase-3 protein increased(P<0.05), the expressions of PI3 K and Akt protein did not change(P>0.05). Compared with the high glucose group, the relative survival rate and the expressions of IGF-Ⅱ, Bcl-2, p-PI3 K and p-Akt in the 5-aza-dc+high glucose group increased(P<0.05); IGF-Ⅱ methylation level, apoptotic rate, Bax and caspase-3 protein expressions decreased(P<0.05), the expressions of PI3 K and Akt protein did not change(P>0.05). Conclusion IGF-Ⅱ methylation mediates trophoblast cells apoptosis induced by high glucose. Inhibition of IGF-Ⅱ methylation can promote proliferation and inhibit apoptosis of trophoblast cells, which may be achieved by up-regulating PI3 K/Akt pathway.

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Objective To explore the interaction between risperidone(RI) and bovine serum albumin(BSA) on the presence of polyinosinic-polycytidylic acid(Poly I:C), and the effects of metal ions(Mn2+,Cu2+,Cr3+,Mg2+,Co3+) on the interaction. Methods The interaction of quenching type and mechanism were determined using UV-visible absorption spectroscopy and fluorescence spectroscopy. Hill curve was used to study the synergistic effect of drug combination. The values of the quenching constant, quenching rate constant, the binding constant and the number of binding sites were calculated according to the Stern-Volmer equation and its modified equation. Thermodynamic parameters and type of interactions were obtained be obtained from Vanʼt Hoff equation. Results In presence of Poly I:C, the fluorescence of BSA was quenched by Poly I:C and RI, which was a dynamic quenching process. The interaction of RI and BSA with Poly I:C was mainly driven by hydrogen bond and Vander Waals force. Location of the primary binding site for Poly I:C and RI was on the sub-domain ⅡA of BSA. When Poly I:C and RI bound to BSA simultaneously, the values of Hill′s coefficients were close to one, which indicated that there was no cooperative effect. The conjugation reaction between Poly I:C-RI and BSA would hardly impact on the BSA conformation, and the binding site was closer to tryptophan residue. Conclusion The addition of Poly I:C played a positive role in enhancing the efficacy of RI. The presence of common metals affected the binding of Poly I:C and RI to BSA. This work will provide scientific reference for their clinical application of RI and Poly I:C combined with medication.

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Objective To investigate the effects of TGFβ1 on the synthesis and degradation of type Ⅰ collagen through the Sp1 signaling pathway. Methods After primary culture of guinea pig scleral fibroblasts, immunohistochemical extrachromosomal detection of Sp1 and type Ⅰ collagen was performed. The mRNA and protein expression of type Ⅰ collagen was detected by qRT-PCR and Western blot method in 4 groups of cells with different treatments. Results Compared with the control group,the expressions of Sp1 protein,type Ⅰ collagen and mRNA increased in the TGFβ1 group(P<0.05),and the expressions in TGFβ1+TGFβ1 inhibitor group were weakened(P<0.05),TGFβ1.The expressions in the +Sp1 inhibitor group were weaker but stronger than those in the TGFβ1+TGFβ1 inhibitor group(P<0.05).Conclusion TGFβ1 can promote the expression of Sp1 protein and type Ⅰ collagen,and thus TGFβ1 can regulate the synthesis and degradation of type Ⅰ collagen through Sp1 signaling pathway.

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Objective To explore the changes of frequency, phenotype and function of peripheral blood mucosal-associated invariant T(MAIT) cells in colorectal cancer patients compared with healthy controls. Methods Peripheral blood mononuclear cells(PBMCs) were isolated from human peripheral blood, which were collected from the healthy donors and colorectal cancer patients, with density gradient centrifugation, and the cells were activated by PMA + ionomycin. The frequency, phenotype and intracellular cytokine production of MAIT cells were determined by flow cytometry. Results ① The frequency and absolute number of peripheral blood MAIT cells with colorectal cancer patients were lower than those in healthy controls(P=0.022,P<0.001).② In the same patient, the frequency and absolute number of peripheral blood MAIT cells before and after chemotherapy were no difference(P=0.507,P=0.743). ③ Compared with healthy controls, the levels of PD-1 and CD69 molecules on peripheral blood MAIT cells surface in colorectal cancer patients were both different(P=0.049,P<0.001), while Tim-3 molecule level was not different(P=0.757). ④ There was no difference in the function of MAIT cells in patients with colorectal cancer compared with healthy controls(P=0.545,P=0.121). ⑤ The frequency and absolute number of MAIT cells were not significantly correlated with clinical tumor stage(P=0.261,P=0.173). Conclusion Compared with healthy controls, the frequency and absolute number of MAIT cells from peripheral blood of colorectal cancer patients decrease, the phenotype change, but the function is no difference. Chemotherapy has no effect on the frequency of MAIT cells in the peripheral blood of colorectal cancer patients. The frequency of MAIT cells is not correlated with clinical tumor stage as well.

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Objective To investigate the effect of miR-223-3 p on cisplatin sensitivity of lung squamous cell carcinoma. Methods After increasing or decreasing(miR-223-3 p mimics, NC-mimics, miR-223-3 p inhibitor, NC-inhibitor) the content of miR-223-3 p in lung squamous carcinoma NCI-H520 and SK-MES-1 cells, the changes of miR-223-3 p in cells were detected by qRT-PCR, half inhibitory concentration(IC50) was detected by CCK-8 assay, apoptosis was detected by flow cytometry(four different treatments were designed:miR-223-3 p mimics+CDDP, NC-mimics, miR-223-3 p inhibitor+CDDP, NC-inhibitor), the migration ability of SK-MES-1 cells was detected by Transwell assay, the proteins of Bax, ZEB1 and E-cadherin in SK-MES-1 cells were detected by Western blot. Results The results of CCK-8 experiment showed that the IC50 of the miR-223-3 p mimics group was lower than that of the control group, and the IC50 of the miR-223-3 p group was higher than that of the control group. Flow cytometry apoptosis detection results showed that the apoptosis rate of miR-223-3 p mimics+ cisplatin(CDDP) group increased, while the apoptosis rate of miR-223-3 p inhibitor+ cisplatin(CDDP) group decreased. Western blot confirmed that ZEB1 protein, Bax and E-cadherin protein increased and decreased respectively after the content of miR-223-3 p in lung squamous cell carcinoma was increased or decreased. Conclusion miR-223-3 p affects the sensitivity of lung squamous cell carcinoma cells to cisplatin as an anticancer gene, and it can be an important molecular target for the treatment of cisplatin resistance in lung squamous cell carcinoma.

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Objective To investigate the effects of Hashimoto′s thyroiditis(HT) on the concentration of glutamate(Glu) in the frontal lobe of pregnant rats and the changes of glutamate transporters. Methods After adaptively feeding for a week, 50 NOD female micewere randomly divided into normal control group(CON group, 20 mice) and Hashimoto thyroiditis group(HT group, 30 mice). HT mouse model was prepared by subcutaneous injection of porcine thyroglobulin in the tail root of HT group. After successful modeling, female mice in each group were caged with healthy NOD males to get pregnant. Serum thyroid stimulating hormone(TSH) levels were determined by ELISA. The levels of serum thyroid peroxidase antibody(TPOAb), thyroglobulin antibody(TgAb) and serum thyroid hormone triiodothyronine(T3) and thyroxine(T4) were determined by electrochemiluminescence immunoassay. The concentration of Glu in the frontal lobe of pregnant rats was detected by ELISA. Western blot assay was used to detect protein expression levels of vesicular glutamate transporter VGLUT 1 and excitatory glutamate transporter EAAT 2 in frontal lobe. RT-qPCR was used to observe the expression of VGLUT1 and EAAT2 mRNA in the frontal lobe of pregnant rats. Results ① Serum thyroid antibody levels(TPOAb, TgAb) in HT group were higher than those in CON group(P<0.05). There was no significant difference in serum T3, T4 and TSH between the two groups; ② The ELISA showed that the glutamate content in the frontal lobe of the HT group was higher than that in the CON group(P<0.05); ③ Western blot method showed that the expression of VGLUT 1 protein in HT group was lower than that in CON group(P<0.05), and the protein expression level of EAAT 2 in the two groups was not statistically significant;④ RT-qPCR showed that the mRNA expression of VGLUT 1 in the frontal lobe of HT group was lower than that in CON group(P<0.05). There was no significant difference in the expression level of EAAT 2 mRNA between the two groups. Conclusion HT can increase the content of Glu in the frontal lobe of pregnant mice, and decrease the expression of the glutamate transporter VGLUT 1 protein, suggesting that HT can cause glutamate metabolism disorders.

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Objective To observe the level of intracellular free Ca2+in cortex and hippocampus of epileptic rats and the effect of low dose gamma knife irradiation on it. Methods 32 Wistar rats were randomly divided into four groups: ① Normal control group; ② gamma knife(GK) control group; ③ pentylenetetrazole(PTZ) group; ④ PTZ+GK group. Epileptic rat model was prepared by continuous intraperitoneal injection of PTZ. Leksell gamma knife system was used to irradiate rats with low dose gamma knife for 12 weeks. The target area was bilateral frontal lobe. On the 12 th week after gamma knife irradiation,the brains of rats were decapitated and neurons of frontal lobe and hippocampus were acutely isolated. The neuron cell suspension was prepared,and then laser scanning confocal microscopy(LSCM) was used to detect the level of free Ca2+in nerve cells. Results Compared with the normal control group,the fluorescence intensity of Ca2+in cortex and hippocampal neurons of PTZ group increased significantly(P<0.05). Compared with PTZ group,the fluorescence intensity of Ca2+in neurons of PTZ+GK group decreased markedly(P<0.05). The fluorescence intensity of Ca2+in neurons of GS control group was slightly higher than that of normal control group,but the difference was not statistically significant(P>0.05). Conclusion The increased Ca2+level of neurons in frontal cortex and hippocampus may be one of the important mechanisms of epilepsy. Low dose of gamma knife irradiation may play a role of anti-epileptic and improve cognitive function by affecting intracellular calcium homeostasis.

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Objective To research the effects of sodium valproate(VPA) on proliferation, migration of human lung adenocarcinoma PC9 cells in different concentrations and its possible mechanism. Methods PC9 cells were divided into control group(0 mmol/L VPA)and experimental groups(2, 4, 8 and 16 mmol/L VPA), and they were cultured in vitro. CCk-8 and scratch test were used to detect the effects of VPA of different concentrations on the proliferation and migration ability of PC9 cells under different time conditions. The effects of VPA on lactate dehydrogenase activity and lactic acid production in different concentrations were detected by lactate dehydrogenase activity assay kit and lactate quantitative assay kit respectively. ELISA was used to detect the expression levels of E-caducin( E-cad) and vimentin( VIM) in cell culture supernatant and in cells. Results CCK-8 result revealed that VPA had a concentration-dependent and time-dependent cell proliferation inhibition effect on PC9 cells(P<0.05). The scratch test result indicated that the migration distances of the experimental groups were smaller than that of the control group(P<0.05),and the migration distance gradually decreased when VPA concentration increased(P<0. 05). LDH activity and LD test results indicated that the activity of LDH and LD production of the experimental groups were significantly lower than that of the control group(P<0.05). LDH activity and LD production gradually decreased when VPA concentration increased(P<0.05). ELISA results indicated that the cell culture supernatant and cells in the experimental groups had more E-cad protein than the control group,and the concentration of E-cad increased gradually when VPA concentration increased(P<0. 05). The cell culture supernatant and cells in the experimental group had less VIM protein than the control group,and VIM protein concentration gradually decreased when VPA concentration increased(P<0.05). Conclusion The proliferation and migration of PC9 cells in vitro can be restrained by VPA,possibly by restraining aerobic glycolysis and epithelial mesenchymal transformation.

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Objective To investigate the effect of resveratrol(Rev) on proliferation and migration of ovarian cancer SKOV-3 cells by regulating IL-6/JAK2/STAT3 signaling pathway. Methods According to drug treatment, the cells were divided into SKOV-3 blank group(CON), Rev low concentration group(Rev-L), Rev medium concentration group(Rev-M) and Rev high concentration group(Rev-H). CCK-8 assay was used to detect the proliferation of SKOV-3 cells. Flow cytometry was used to detect the apoptosis of SKOV-3 cells. Healing assay was used to detect the migration of SKOV-3 cells. Transwell test was used to detect the invasion of SKOV-3 cells. Western blot assay was used to detect the expression levels of metastasis-related proteins, apoptosis-related proteins and IL-6/JAK2/STAT3 signaling pathway proteins in the each group. Results Compared with CON group, the apoptosis of SKOV-3 cells increased after Rev treatment, the proliferation, migration and invasion ability were inhibited; B lymphoma-2(Bcl-2) expression level was decreased; Bcl-2 family apoptotic proteins(Bax) and activated Caspase-3(cleaved Caspase-3) expression levels were increased; transfer related protein Ca2+dependent cell adhesion moleculue family-E(E-cadherin) expression level was increased, Ca2+dependent cell adhesion moleculue family-N(N-cadherin) and Vimentin expression levels were increased; and the activity of IL-6/JAK2/STAT3 signaling pathway decreased. Conclusion Rev can down-regulate the activity of IL-6/JAK2/STAT3 signaling pathway, promote the apoptosis of SKOV-3 cells, and inhibit the proliferation, migration and invasion of SKOV-3 cells.

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Objective To explore the feasibility of the adaptive statistical iterative reconstruction-V(ASiR-V) in piglet abdominal CT volume scanning,and the optimal low dose level to maintain image quality,so as to provide reference for optimizing combination of pre-and post-ASiR-V percentage in children's abdominal CT scan. Methods A total of 10 standard piglets were selected and scanned to obtain plain and enhanced images under different preASiR-V conditions,and the corresponding images were obtained using post-ASiR-V reconstruction. CT values,noises,subjective scores,and radiation doses of unenhanced and enhanced abdominal tissues were analyzed respectively,and the data of each group were statistically analyzed. Results With the increase of pre-ASiR-V,the radiation dose in piglets gradually decreased(P<0.05). In the same pre-ASiR-V group and with the increase of postASiR-V,SD values gradually decreased,and the CNR values did not change or increase. In plain scan images,the subjective score of the images with 40% pre-ASiR-V combined 60% post-ASiR-V was similar to 0% pre-ASiRV combined 60% post-ASiR-V,and the radiation dose was reduced by 31. 61%. In the enhanced images,the subjective score of the images with 60% pre-ASiR-V combined 60% post-ASiR-V was similar to 0% pre-ASiR-V combined 60% post-ASiR-V,and the radiation dose was reduced by 48. 9%. Conclusion ASiR-V can significantly reduce the radiation dose without affecting image quality,and it is recommended to use 40% pre-ASiR-V combined with 60% post-ASiR-V for children's abdominal CT plain scan,and 60% pre-ASiR-V combined with 60% postASiR-V for children's abdominal CT enhanced scan,which is beneficial to theoretically reduce the damage of radiation dose.

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Objective To explore effects of interleukin-2(IL-2) and IL-2 receptor(IL-2 R) on Fas/FasL expression and T cell apoptosis in renal transplantation immune tolerance induced by bone marrow mesenchymal stem cells(MSCs). Methods Thirty BALB/c mice with immunodeficiency and thirty C57 BL/6 mice were randomly divided into 3 groups(control group, transplant group, IL-2 group), 10 the former mice and 10 the latter mice in each group. In transplant group, kidneys of C57 BL/6 mice were transplanted into BALB/c mice. After 24 h, normal MSCs were intravenously injected into transplant receptors. In IL-2 group, IL-2 mRNA and target gene were introduced into MSCs, and then they were intravenously injected into transplant receptors. The expression of IL-2 mRNA and IL-2 R mRNA in mice was detected. The levels of IL-2, transforming growth factor β1(TGF-β1), soluble apoptosis-related factors(sFas) and their ligands(sFasL) in mice blood were detected by ELISA. Western blot was applied to detect the expression of JAK, STAT3 and p-STAT3 in renal tubular epithelial cells. Results Compared with control group, the expression of IL-2 mRNA and IL-2 R mRNA, levels of serum TGF-β1, sFas and sFasL, and the expression of JAK and p-STAT3 proteins in renal tubular epithelial cells were increased in transplant group(P<0.05). There was no statistical difference in STAT3 in renal tubular epithelial cells(P>0.05). Compared with transplant group, the expression of IL-2 mRNA and IL-2 R mRNA, levels of serum TGF-β1, sFas and sFasL, and the expression of JAK and p-STAT3 proteins in renal tubular epithelial cells were increased in in IL-2 group(P<0.05). There was no statistical difference in STAT3 in renal tubular epithelial cells(P>0.05). Conclusion Il-2 can enhance rejection to transplanted kidney by promoting the expression of Fas/FasL and mediating apoptosis of T cells.

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Objective To explore the targeting relationship between miR-106 a-5 p and ERK2 and its effect on cisplatin resistance of gastric cancer cell line MGC-803. Methods RT-PCR was used to detect the expression of miR-106 a-5 p and ERK2 in gastric cancer cell line MGC-803 and drug-resistant cell line MGC-803/DDP. The luciferase reporter assay was used to determine the targeting relationship between miR-106 a-5 p and ERK2. The miR-106 a-5 p mimic and overexpression vector pcDNA3.1-ERK(pc-ERK2) were transfected into drug-resistant cell line MGC-803/cisplatin(DDP), respectively. The cells were divided into control group, miR-106 a-5 p mimic group, ERK2 group and mimic+ERK2 group.Then MTT&EDU were used to measure cell proliferation, hochest33342 was used to observe cell apoptosis, Transwell was used to detect cell invasion. Finally, the expression levels of E-cadherin(E-cad) and N-cadherin(N-cad) in each group of cells were detected by Western blot. Results The expression of miR-106 a-5 p in gastric cancer cell line MGC-803 was significantly higher than that in drug-resistant cell line(P<0.05), while the expression of ERK2 was lower than that in drug-resistant cell line(P<0.05). Secondly, luciferase reporter assays had shown that miR-106 a-5 p targets inhibited ERK2 expression. After transfection with miR-106 a-5 p&pc-ERK2, compared with the control group, the viability, cell proliferation and cell invasion number of ERK2 group significantly increased(P<0.05), and the number of apoptosis significantly decreased(P<0.05); the viability, cell proliferation and cell invasion of the miR-106 a-5 p mimic group were significantly lower than those of the control group(P<0.05), and the number of apoptosis significantly increased(P<0.05). Compared with the ERK2 group, the viability, cell proliferation and cell invasion of the mimic+ERK2 group significantly decreased(P<0.05), and the number of apoptosis significantly increased(P<0.05). Finally, compared with the control group, the expression of E-cad in the miR-106 a-5 p mimic group significantly increased(P<0.05), while the expression of N-cad significantly decreased(P<0.05); meanwhile the expression of E-cad in the ERK2 group significantly decreased(P<0.05), while the expression of N-cad was significantly increased(P<0.05). Compared with the ERK2 group, the expression of E-cad in the mimic+ERK2 group significantly increased(P<0.05), while the expression of N-cad significantly decreased(P<0.05). Conclusion Mir-106 a-5 p can target down-regulate the expression of ERK2 and reduce the drug resistance of gastric cancer cells. It is expected that mir-106 a-5 p can be combined with cisplatin chemotherapy and improve the efficacy of cisplatin chemotherapy.

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Objective To explore the protection of ketogenic diet on hippocampal neuronal injury in mice exposure to high altitude hypoxia. Methods C57 BL/6 mice were randomly allocated into four groups: a normal oxygen control(NC) group, a normal oxygen with ketogenic diet(NK) group, a hypoxia control(HC) group, a hypoxia with ketogenic diet(HK) group(n=11). The hypobaric oxygen chamber was used to simulate high altitude(6 000 m)hypoxic environment, operating time 24 h/d, the control ratio of day to night approximately 12 h:12 h. HC group and HK group were given standard diet and ketogenic diet respectively for 3 weeks, and then were exposed to high-altitude hypoxia circumstances for 6 days. NC group and NK group were raised outside the hypobaric oxygen chamber after 3-week standard diet and ketogenic diet respectively. The pathological morphology of hippocampal neurons was examined by Nissl staining. Expressions of mitochondrial fusion protein 1(MFN1), mitochondrial fission protein 1(FIS1) were assayed by Western blot. Changes of mitochondrial oxygen consumption rate(OCR) in brain was detected by Seahorse energy metabolism analyzer. Results Compared with NC group, HC group showed the pathological damage such as decreased hippocampal neurons, widening intercellular spaces and nuclear pyknosis. Compared with HC group, HK group showed an increase of hippocampal neurons, a gradual alignment of cells and a decrease of nuclear pyknosis. The expression of FIS1 increased while MFN1 decreased in HC group compared with NC group. Compared with HC group, the expression of MFN1 increased while FIS1 decreased in HK group. Seahorse results showed that the mitochondrial OCR in brain of HC group decreased compared with NC group, which increased in HK group compared with HC group. Conclusion High altitude hypoxia causes damage of hippocampal neurons in mice. Ketogenic diet can improve the damage of hippocampal neurons in mice exposure to high altitude hypoxia.

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Objective To study the key regulatory factors for in situ liquid phase reduction of nano-silver on dopamine surface and to evaluate the antimicrobial properties of the prepared surface. Methods The dopamine-modified micro-nanoporous titanium discs were immersed in silver nitrate solution with various concentrations and pH values to prepare different nano-silver modified surfaces. The surface properties were evaluated by scanning electron microscopy, atomic emission spectrometry and static water contact angle. Antibacterial activity was evaluated by the turbidity method, coating plate method, inhibition zone and live/dead bacterial staining. Results Compared with acidic and neutral environments, alkaline environments significantly promoted the deposition of silver nanoparticles, and the deposited nano-silver were smaller in size and more evenly distributed. The quantity of silver loaded on the surface was positively correlated with the concentration of silver nitrate in the solution. The antibacterial ability of the constructed surface was greatly enhanced with the increase of pH and silver concentration. Conclusion The pH value of solution has a stronger effect on in situ reduction of nanosilver compared to the concentration of silver ions, and high pH value is conducive to the preparation of good antibacterial surface for inhibiting infection.

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Objective To investigate the effect of CD147 on sensitivity of prostate cancer cells to docetaxel(DOC). Methods The experiment included negative control group(PC-3/Scramble) and CD147 shRNA group(PC-3/shCD147). PC-3/Scramble and PC-3/shCD147 cells were treated with DOC for 72 h. The cell proliferation was detected by MTT assay. The cell apoptosis was determined by flow cytometry. The protein expressions of β-catenin and P-glycolprotein(P-gp) were detected by Western blot. Results IC50values of DOC treatment in PC-3/Scramble cells(12.47±1.71) μmol/L were higher than those in PC-3/shCD147 cells(2.38±0.13) μmol/L. The 5 μmol/L DOC induced apoptosis in PC-3/Scramble cells(8.75±0.75)% was lower than that in PC-3/shCD147 cells(16.1±0.95)%(P<0.01). The expression of β-catenin and P-gp protein in PC-3/shCD147 cells was significantly lower than that in PC-3/Scramble cell(P<0.01). Wnt pathway agonist LiCl-treated PC-3/shCD147 cells showed the increase in cell survival(P<0.01) and expression levels of β-catenin and P-gp(P<0.01) compared with PC-3/shCD147 cells. Conclusion CD147 decreases the sensitivity of prostate cancer cells to DOC via Wnt/β-catenin pathway.

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Objective To investigate the effect of N-myc downstream regulatory gene 1(NDRG1) overexpression on the pro-angiogenic ability of trophoblastic cells HTR-8/SVneo under hypoxic condition. Methods HTR-8/SVneo cells with stable overexpression of NDRG1 were established by infecting with NDRG1 overexpression lentivirus, and then hypoxia(1% O2) was used to intervene for 24 h. qRT-PCR was used to detect the mRNA expression levels of NDRG1, vascular endothelial growth factor(VEGF) and solunle vascular endothelial growth factor receptor-1(sFlt-1). The protein expression levels of NDRG1, MMP-2 and MMP-9 were detected by Western blot. The protein contents of VEGF and sFlt-1 in the cell culture supernatant were detected by ELISA. The ability of promoting pro-angiogenesisin vitrowas tested by tubule formation assay. The invasion ability of cells was detected by transwell assay. Results Hypoxia could induce the mRNAs expression of NDRG1, VEGF and sFlt-1 in HTR-8/SVneo cells, promote the protein expression of NDRG1 and the protein secretions of VEGF and sFlt-1, inhibit the proteins expression of MMP-2 and MMP-9, and reduce the pro-angiogenesis and invasion ability of cells in vitro. Overexpression of NDRG1 promoted the mRNA expression and protein secretion of VEGF in HTR-8/SVneo cells under hypoxic condition, and inhibited the mRNA expression and protein secretion of sFlt-1. In addition, overexpression of NDRG1 also increased the protein expression levels of MMP-2 and MMP-9 in HTR-8/SVneo cells under hypoxic condition, and promoted the pro-angiogenesis and invasion ability of cells in vitro. Conclusion Overexpression of NDRG1 can enhance the pro-angiogenesis and invasion ability of trophoblast cells under hypoxic condition, and its mechanism may be related to the promotion of VEGF expression and inhibition of sFlt-1 expression.

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Objective To study the effects of mitochondrial division inhibitor(Mdivi-1) on behavior, dynamin-related protein 1(DRP1) and equilibrative nucleoside transporter-1(ENT1) proteins in hippocampus of epileptic rats. Methods Twenty male SD rats were randomly divided into normal group, model group, DMSO group and Mdivi-1 group(5 rats in each group). Behavioral changes of rats in each group were observed, and brain tissue structure changes were observed by transmission electron microscopy. The DRP1 expression in hippocampus of rats was detected by immunohistochemistry and immunofluorescence. Changes of DRP1 and ENT1 proteins in hippocampus of rats in each group were detected by Western blot. Results Compared with model group or DMSO group, the latency of epileptic seizures in Mdivi-1 group increased and the frequency of epileptic seizures decreased(P<0.05). The results of electron microscopy showed that the epilepsy group had partial disintegration of inner and outer membranes, obvious destruction and swelling of mitochondria, while the mdivi-1 group had less damage. Immunohistochemical results showed that the number of DRP1 positive cells in hippocampus increased in model group while decreased in Mdivi-1 group(P<0.05). Immunofluorescence showed that DRP1 was expressed in hippocampal neurons. Western blot results showed that the expression of ENT1 and DRP1 protein in DMSO and model group was higher than that in normal control group,while the expression of ENT1 and DRP1 protein in Mdivi-1 group was lower(P<0.05). Conclusion Mdivi-1 can restrain DRP1 and regulate the dynamic balance of mitochondria,thus affecting the expression of ENT1,which has neuroprotective effect on hippocampal neurons.

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Objective To study the effect of TCM intervention on differential proteomics of small intestine in atherosclerosis(AS) bama pig with spleen deficiency and phlegm turbid by iTRAQ technique. Methods Ten healthy half-year-old male Guangxi Bama miniature pigs were randomly divided into model group and TCM group, five pigs in each group. Total proteins of small intestine was isolated, labeled with iTRAQ reagent and detected by mass spectrometry. The differentially expressed proteins were analyzed by software, and the proteomics differences between the model group and the treatment group were analyzed by LC-ESI-MS/MS method. The significantly enriched proteins were selected GO function annotation, enrichment and KEGG pathway enrichment. Results A total of 189 different proteins were screened in the treatment group compared with the model group, including 70 up-regulated proteins and 119 down-regulated proteins. GO enrichment analysis showed that these differential proteins were mainly concentrated in biological processes such as binding, catalytic activity, transport activity and structural molecular activity. It was concentrated in cells, cell components, organelles, cell membranes and extracellular regions. The molecular functions such as cell process, biological regulation, biological process regulation, metabolic process, stimulation response and localization were enriched.Analysis of the KEGG pathway showed that cell adhesion molecular pathways had the most significant changes, with significant changes in phagocytosis, prominent vesicle circulation, amino acid biosynthesis, insulin secretion, PPAR signaling pathways, fat digestion and absorption, and bile acid secretion pathways closely related to lipid metabolism.In addition, oxidative phosphorylation and other pathway proteins closely related to energy metabolism also changed. Conclusion The differential proteomics changes of small intestine in bama pig with spleen deficiency and phlegm turbid under the intervention of TCM may be closely related to cellular inflammatory response, lipid metabolism and bile acid secretion.Intestinal digestion and absorption in the state of spleen deficiency and high fat are abnormal, and the cellular inflammatory reaction may be closely related to the formation of AS. The therapeutic targets of TCM intervention may be in the above directions.

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Objective To investigate the efficacy and safety of rivaroxaban for 100 U unfractionated heparin per kilogram body weight in anticoagulation treatment without activated coagulation time(ACT) monitoring before catheter ablation for patients with atrial fibrillation, and to analyze the efficacy and safety of rivaroxaban after catheter ablation of atrial fibrillation.Methods Ninety-eight patients were selected for catheter ablation of atrial fibrillation, among whom 45 patients had paroxysmal atrial fibrillation and 53 patients had persistent atrial fibrillation. The patients were divided into two groups according to different anticoagulant drugs administered preoperatively. One group received rivaroxaban in anticoagulant treatment, known as the rivaroxaban group, with a total of 31 cases. Another group received warfarin, known as the warfarin group, also as the control group, a total of 67 cases. Both groups were administered uninterrupted anticoagulant drugs before catheter ablation. According to clinical experience, all subjects received intravenous unfractionated heparin with 100 u per kilogram body weight at the beginning of ablation, with another 1 000 U unfractionated heparin added for each hour during catheter ablation. 17 ml/min cold saline infusion with 1 ∶1 unfractionated heparin was applied during catheter ablation. ACT was not monitored in all subjects. The postoperative anticoagulation regimen was the same as preoperative for at least 3 months. The postoperative follow-up consists of readmission follow-up,outpatient follow-up and telephone follow-up,and the incidences of intraoperative bleeding and thromboembolic events were assessed in two groups during operation,hospitalization and 3 months after catheter ablation. Results The baseline data of two groups showed no statistical significance except body weight. No pericardial tamponade,TIA,cerebral embolism,cerebral hemorrhage and puncture site hemorrhage were observed. Cerebral embolism occurred in one case in rivaroxaban group on the first day after catheter ablation,which was not detected in warfarin group. Statistical results showed no difference in the incidence of cerebral embolism between the two groups during hospitalization,and no other adverse events occurred during hospitalization. No statistically significant difference was detected in the incidence of puncture site bleeding,gingival bleeding and gastrointestinal bleeding between the two groups during 3 months' follow-up. Intracerebral hemorrhage occurred in one case in warfarin group,which was not found in rivaroxaban group. No statistically significant difference was detected in the incidence of intracerebral hemorrhage between the two groups,and no thromboembolic events occurred in two groups during 3 months' follow-up. Conclusion Rivaroxaban is safe and effective for 100 U unfractionated heparin per kilogram body weight in catheter ablation of atrial fibrillation without ACT monitoring in patients. The application of uninterrupted oral rivaroxaban is feasible for anticoagulation after catheter ablation of atrial fibrillation,and its safety and efficacy are not inferior to those of uninterrupted warfarin therapy.

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Objective To study the expression and clinical significance of long non-coding RNA FEZ family(lncRNA FEZF1-AS1) in glioma tissues,and to explore the biological function of FEZF1-AS1 in gliomas. Methods qRT-PCR was used to detect the expression of FEZF1-AS1 in 60 glioma tissues and 40 normal brain tissues,and the relationship between the expression of FEZF1-AS1 in glioma tissues and clinical pathological parameters was analyzed. Kaplan-Meier survival curve analyzed the effect of FEZF1-AS1 expression on patient prognosis. FEZF1-AS1 shRNA transfected glioma cell line U87 inhibited the expression of FEZF1-AS1,MTS assay was used to detect cell proliferation. Boyden assay was used to detect cell invasion. TOP/FOP reporter gene detection was used to detect Wnt/β-catenin signaling pathway. Western blot was used to detect the expression of β-catenin protein in cells. Results The expression of FEZF1-AS1 in glioma tissues was higher than that in normal brain tissues. The high expression of FEZF1-AS1 was associated with WHO clinical classification. The glioma patients with high FEZF1-AS1 expression had shorter survival time. After interfering with the expression of FEZF1-AS1 in U87 cells,the proliferation and invasion ability of cells decreased,the luciferase activity of TOP/FOP reporter gene decreased,and the protein expression of β-catenin decreased. Conclusion The expression of FEZF1-AS1 is up-regulated in glioma tissues,and its expression level is related to WHO clinical grade and poor prognosis. Interfering with the expression of FEZF1-AS1 in glioma cells may inhibit cell proliferation and invasion by regulating Wnt/β-catenin signaling pathway.

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Objective To investigate the expression and significance of ferroportin(FPN) in hepatocellular carcinoma. Methods The expression of FPN in serum of 41 healthy controls, 48 chronic liver disease patients and 80 hepatocellular carcinoma patients was detected by ELISA, and the correlation between the degree of FPN in hepatocellular carcinoma group and Child-pugh classification, Barcelona(BCLC) staging of hepatocellular carcinoma was analyzed. 46 cases of liver tissue were collected, and the iron deposition and FPN expression of liver tissue were detected by Prussian blue iron staining and immunohistochemistry. Results In serum, there was no significant difference between healthy control group and chronic liver disease group(P>0.05), but the expression of FPN in hepatocellular carcinoma group was significantly higher than that in healthy control group(P<0.001) and chronic liver disease group(P<0.05). The degree of FPN was positively correlated with the grade of Child-pugh, staging of BCLC(P<0.05) and metastasis, but not with tumor size(P>0.05) and number of nodules(P>0.05). The deposition of blue iron particles was found in patients with hepatocellular carcinoma, and the expression of FPN in liver cancer tissue group was significantly higher than that in benign liver disease tissue group(P<0.05). Conclusion FPN is highly expressed in patients with hepatocellular carcinoma, indicating that FPN may play an important role in the occurrence and development of hepatocellular carcinoma, and it can be used as a new target for immunotherapy of hepatocellular carcinoma.

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Objective To observe the impact of risk factors and imaging changes of pontine infarction on short-term prognosis.Methods 111 patients with pontine infarction were divided into good prognosis group(76 cases) and poor prognosis group(35 cases) according to the mRS score at 90 days follow-up.The gender,age,smoking,complications,systolic blood pressure,diastolic blood pressure,fasting blood glucose,homocysteine(Hcy) and glycosylated hemoglobin(HbA1c) levels were compared between the two groups.Logistic regression analysis was used to analyze the factors affecting the short-term prognosis of ponstine infraction in two groups.Results There were significant differences in diabetes history(n=24,31.6%; n=18,51.4%; P=0.045),stroke history(n=18,23.7%; n=19,54.3%; P=0.001),hypertension,diabetes and stroke history(n=3,3.9%; n=9,25.7%; P =0.001),and HbA1c level(5.85 ± 1.59; 7.20 ± 2.32; P=0.005) between the good prognosis group and the poor prognosis group.Logistic multivariate regression analysis showed that history of diabetes(OR=2.518,95% CI:1.018-6.232,P=0.046),history of stroke(OR=3.822,95% CI: 1.537-9.508,P=0.004),infarct in the anterior medial area(OR=0.149,95% CI: 0.032-0.699,P=0.016) were independent risk factors for the prognosis of pons infarction.Conclusion The short-term prognosis of pontine infarction is related to the risk factors of blood vessels and the location of the infarct.The prognosis of patients with diabetes and stroke is worse.The prognosis of patients with pontine infarction whose infarct is located in the anterior medial area is better.

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Objective To investigate the role of CCL23-CCR1 axis in progression of epithelial ovarian cancer. Methods Serum level of 441 kinds of chemokines was detected using RioBiotech antibody array in 6 patients with epithelial ovarian cancer and 4 normal adult females. Candidate biomarker was identified by SPSS analysis. We identified CCL23 as the differentially expressed protein. Serum levels of CCL23 in 22 normal adult females and 36 epithelial ovarian cancer patients were detected by ELISA. qRT-PCR was used to detect the expression of CCL23 mRNA in 6 normal adult ovarian tissues and 6 epithelial ovarian cancer tissues. Immunohistochemistry was used to detect the expression of CCR1 in situ lesions of 20 cases of Stage Ⅰ, 20 cases of Stage Ⅱ, 40 cases of Stage Ⅲ and 20 cases of Stage Ⅳ epithelial ovarian cancer. Results The concentrations of 441 cytokines were detected using antibody array, and CCL23 concentration was(21 924.20±2 647.89)pg/ml in normal control group while(44 902.00±2 064.03)pg/ml in patients with epithelial ovarian cancer(t=-2.693,P=0.041), the difference was statistically significant. Serum levels of CCL23 were(1 157.80±457.40)pg/ml in 22 normal adult women while(1 654.90±849.00) pg/ml in 36 cases of epithelial ovarian cancer patients(t=-0.244 3,P=0.018 8), the difference was statistically significant. The expression of CCL23 mRNA in epithelial ovarian cancer tissue was significantly higher than that in normal ovarian tissue(t=-7.515,P=0.006). The expression of CCR1 in situ lesions of epithelial ovarian cancer using immunohistochemistry demonstrated that ovarian surface epithelial(OSE) presented negative expression of CCR1. In contrast, the expression of CCR1 in 60 cases of stage Ⅲ/Ⅳ was higher than 40 cases of stage Ⅰ/Ⅱ. Conclusion CCL23-CCR1 axis may play a role in the progression of epithelial ovarian cancer.

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Objective To investigate the expression of RNA binding protein Poly(rC) binding protein 2(PCBP2) in colorectal cancer tissues and its regulatory effect on proliferation and clone formation of colorectal cancer cells. Methods Immunohistochemistry was used to detect the expression of PCBP2 in 50 pairs of colorectal cancer and its relationship with clinical pathological data. The effect of PCBP2 expression on survival time was observed during four years of follow-up. Specific small interfering RNA targeting PCBP2 was transfected into HCT116 cells by liposome-mediated transfection,and the effect of PCBP2 on proliferation and clone formation of colorectal cancer cells was detected by CCK-8 proliferation assay and clone formation assay. Results Compared with the normal adjacent mucosa to the cancer,the expression of PCBP2 in colorectal cancer was significantly increased(P=0.001),and was associated with lymph node metastasis(P=0.031) and tumor stage(P=0.045). But there was no significant correlation between the PCBP2 expression level and gender,size,cell differentiation(P>0.05). Patients with high expression of PCBP2 survived shorter time of recurrence-free survival(P=0.039) or overall survival(P=0.037) than patients with low PBCP2 expression. After inhibiting the expression of PCBP2,the proliferation(P<0.05) and clone forming ability(P<0.05) of colorectal cancer cells were significantly attenuated. Conclusion PCBP2 is highly expressed in colorectal cancer tissues. The high expression of PCBP2 is closely related to clinical grade. Down-regulation of PCBP2 expression can inhibit the proliferation and clone formation of colorectal cancer cells,providing a new target for prognosis evaluation and effective treatment for colorectal cancer.

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Objective To evaluate the prognostic value of infarcted zone Tp-ec and Tp-e/QT in patients with St-segment elevation myocardial infarction. Methods A retrospective analysis was made of 281 patients diagnosed as STEMI. Among them, 36 were in the main adverse cardiac events(MACE) group and 245 were in the non-MACE group. The differences of Tp-ec and Tp-e/QT values before and after percutaneous coronary intervention(PCI) were compared between the two groups. The risk factors and predictors of MACE in STEMI patients were screened by binary Logistic regression analysis and ROC analysis. Results Compared with non-MACE group, MACE group had higher age, faster heart rate, longer door-to-balloon time, higher proportion of women and multiple vessel lesions, lower TIMI blood flow grade 3 ratio and lower left ventricular ejection fraction(LVEF). Univariate analysis showed that the Tp-ec and Tp-e/QT values of MACE group before and after PCI were higher than those of non-MACE group(P<0.05). Multivariate binary Logistic regression analysis showed that Tp-e/QT(OR=1.238,P<0.001), LVEF(OR=0.924,P=0.005) and door-to-balloon time(OR=1.007,P=0.018) were independent risk factors for MACE in STEMI patients before PCI. The area under ROC curve(AUC) was 0.817(95%CI0.763～0.864) and 0.889(95%CI0.843～0.926) respectively. Z test showed that the AUC of preoperative Tp-e/QT was significantly different from that of preoperative Tp-ec, postoperative Tp-ec and postoperative Tp-e/QT(P<0.05). Conclusion Preoperative Tp-e/QT is an independent risk factor for nosocomial MACE in STEMI patients. Preoperative Tp-ec and Tp-e/QT have higher predictive value for MACE.

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Objective To investigate the promoter methylation level of the cytokine signaling 3(SOCS3) inchildren with acute lymphoblastic leukemia(ALL), and to explore its correlation with the treatment outcome and prognosis for ALL children. Methods The SOCS3 mRNA and methylation levels in bone marrow mononuclear cells were detected in 83 cases of newly diagnosed ALL at initial diagnosis, completely remission, relapse and 21 healthy control children through reverse transcription quantitative polymerase chain reaction(qRT-PCR) and methylation-specific PCR, respectively. SPSS 17.0 software was employed for statistical analysis to analyze the relationship between the level of SOCS3 mRNA expression and the methylation level of SOCS3 gene, the relationship between the methylation level of SOCS3 and the treatment outcome of the children with ALL. Meanwhile, The methylation level of SOCS3 gene in children with ALL was classified into high or low level group by the median method. The correlations of SOCS3 methylation status with the cumulative incidence of relapse rate(CIR) and overall survival rate(OS) were compared. Results The SOCS3 mRNA expression levels in initial diagnosis group and relapse group were remarkably lower than that in healthy control group(P<0.05). The methylation level of SOCS3 in ALL children was evidently higher than that in healthy control group(P<0.05). The results of 42 months follow-up showed that the CIR in high level group was dramatically higher than that in low level group(P<0.05), but there was no significant difference in the OS between these two groups(P>0.05). Conclusion SOCS3 methylation leads to low expression of SOCS3, and monitoring the SOCS3 methylation level can help to understand the treatment outcome and prognosis for ALL children.

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To construct RAW264.7 cell line with G-protein-coupled receptor 41(gpr41) gene knockout by CRISPR-Cas9 gene editing technique. Lentivirus was used to transfect Cas9 protein into macrophages. gRNA targetinggpr41 gene was designed to target the functional region ofgpr41 gene, and pLenticrisprV2 recombinant plasmid transformed DH5α competent cells were constructed to screen out recombinant sequencing to verify the effectiveness of gRNA. The plenticrisprV2-gpr41-gRNA recombinant plasmid was transfected with the macrophage line RAW264.7 using transfection reagent. The expression ofgpr41 gene was detected by Western blot. Sequencing results confirmed the successful construction of pLenticrisprv2-gpr41-gRNA recombinant plasmid.Western blot selection of recombinant plasmid pLenticrisprv2-gpr41-gRNA 3 transfected RAW264.7 cell No. 13 monoclonal strain showed no expression of GPR41 protein. CRISPR-Cas9 gene editing technique was used to successfully construct a macrophage line withgpr41 gene knockout,which laid the foundation for studying the mechanism ofgpr41 gene in RAW264.7 cells.

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To investigate the expression difference of circRNA in placentas between normal pregnant women and patients with severe preeclampsia(sPE). Placental tissues were obtained from three pregnant women with sPE and three normal pregnant women. The expression of circRNA in the two groups was detected by RNA-seq technique analysis. The miRNA targets of circRNAs were predicted using miRanda software. There were 3 007 circRNAs expressed in both the experimental group and the control group. Among them, 89 circRNAs were differentially expressed, including 44 up-regulated genes and 45 down-regulated genes. Most of these differentially expressed circRNAs had binding sites for has-let-7, miR-100, miR-101, and miR-103 family members. The results indicate that these dys-regulation of circRNA in placental tissue may be involved in the pathogenesis and pathophysiology of sPE.

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To investigate the risk factors of primary Sjögren′s syndrome with pulmonary arterial hypertension(pSS-PAH) for early diagnosis and treatment, a total of 438 patients with primary Sjögren′s syndrome(pSS) were selected. Patients were divided into two groups according to pulmonary arterial pressure detected by cardiac ultrasonography. The clinical and laboratory characteristics between the two groups were compared. Among 438 patients, 80 had PAH, the incidence rate was 18.3%.Age of pSS-PAH was 22～77(59.9±13.8) years old, and pulmonary arterial pressure was between 5.33～15.19 kPa. Multivariate logistic regression analysis suggested that interstitial pneumonia, age, and initial pulmonary symptoms were the risk factors for pSS-PAH. In these cases, cardiac ultrasonography should be performed as soon as possible, early diagnosis and treatment can improve the prognosis of patients.

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A descriptive cohort study was conducted to analyze purine metabolism in saliva samples from 178 patients with gastritis and gastric cancer. 167 differential metabolites were identified by ultra-high performance liquid chromatography-mass spectrometry(UHPLC-QTOFMS). Xanthosine, Cyclic GMP, uric acid and 2′-Deoxyadenosine 5′-monophosphate were screened out by Studentttest and orthogonal partial least square analysis. ROC curve was used to evaluate the inter group discrimination effect of diagnostic markers. Fisher discriminant analysis was used to establish the diagnostic function model, and the sensitivity and specificity of the diagnostic model were obtained as 83.1% and 63.3% respectively by leave-one-out cross validation. The screening of purine metabolites in saliva has great potential in distinguishing early gastric cancer from gastritis and screening early gastric cancer.

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To investigate the role of prostate-specifific antigen isoform 2(p2 PSA) and its related marker prostate health index(PHI) in the diagnosis of prostate cancer, and to further study the effect of different levels of preoperative PHI on the biochemical recurrence(BCR) of prostate cancer. Serum p2 PSA,total prostate specifific antigen(tPSA) and free prostate specifific antigen(fPSA)were determined in 38 patients with prostate cancer and 69 patients with benign prostatic hyperplasia(BPH) confirmed by pathology.The BCR of PCA patients after 12 months was followed up. The optimal cutoff value of PHI was determined by using the ROC curve. The patients were divided into low PHI(PHI≤71.28) and high PHI(PHI>71.28) groups. The recurrence rate of BCR and the effect of different levels of PHI on BCR after surgery was compared. Age,tPSA,fPSA,p2 PSA and PHI between prostate cancer group and BPH group had significant statistical differences(P<0.05). ROC curve analysis showed that the areas under curves(AUC) of tPSA, p2 PSA, f-PSA and PHI in the diagnosis of prostate cancer were 0.637(95%CI: 0.529～0.745), 0.731(95%CI: 0.630～0.833) and 0.845(95%CI: 0.770～0.920) respectively. The diagnostic efficacy of PHI and p2 PSA in prostate cancer was better than that of fPSA,which had higher diagnostic value(AUC>0.7). The results showed that the biochemical recurrence of high PHI group was significantly higher than that of low PHI group after 12 months of follow-up, and the difference was statistically significant(P=0.015). The diagnostic efficacy of PHI for prostate cancer is better than those of tPSA and fPSA,which can be used as an auxiliary diagnostic method for prostate cancer. The PHI=71.28 might be the critical value for evaluating the biochemical recurrence rate of prostate cancer and it provides some valuable references for predicting the biochemical recurrence rate of prostate cancer before operation.